Induced collagen cross-links enhance cartilage integration.
Induced collagen cross-links enhance cartilage integration.
复制标题
DOI：
10.1371/journal.pone.0060719
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Hu JC
中科院分区：
文献类型：
--
作者：
Athens AA;Makris EA;Hu JC
关节软骨不能融合主要是由于界面缺乏交联物和活细胞所致。这项研究的目的是验证一种假设，即赖氨酰氧化酶，一种形成胶原交联物的金属酶，将有效地促进天然软骨表面和组织工程软骨表面之间的整合。为了验证这些假说，通过自组装过程合成的工程软骨结构以及天然软骨被植入天然软骨环中，并用赖氨酰氧化酶处理不同的时间。在两组患者中，赖氨酰氧化物酶的应用导致了软骨界面上更大的明显硬度，是对照组的2-2.2倍。构建-天然赖氨酸氧化酶组的表观强度也有统计学上的显著增加，在这里定义为拉伸测试中观察到的最高峰值应力。组织学显示，赖氨酰氧化酶处理组的软骨界面缝隙缩小，尽管这本身并不足以表明退火组。然而，当形态数据和力学数据结合在一起时，赖氨酰氧化酶处理的时间越长，界面就越完整。虽然还需要进一步的数据来确认作用机制，但整合的增强可能是由于赖氨酰氧化酶诱导的吡啶酚交联物。这项研究表明，赖氨酰氧化酶是一种有效的试剂，可以促进天然到天然和天然到工程软骨之间的整合。界面强度增加的事实表明，交联剂应该在解决软骨结合这一难题上发挥重要作用。未来的研究必须检查剂量、给药方案和细胞对赖氨酰氧化酶的反应，以优化其应用。
Articular cartilage does not integrate due primarily to a scarcity of cross-links and viable cells at the interface. The objective of this study was to test the hypothesis that lysyl-oxidase, a metalloenzyme that forms collagen cross-links, would be effective in improving integration between native-to-native, as well as tissue engineered-to-native cartilage surfaces. To examine these hypotheses, engineered cartilage constructs, synthesized via the self-assembling process, as well as native cartilage, were implanted into native cartilage rings and treated with lysyl-oxidase for varying amounts of time. For both groups, lysyl-oxidase application resulted in greater apparent stiffness across the cartilage interface 2–2.2 times greater than control. The construct-to-native lysyl-oxidase group also exhibited a statistically significant increase in the apparent strength, here defined as the highest observed peak stress during tensile testing. Histology indicated a narrowing gap at the cartilage interface in lysyl-oxidase treated groups, though this alone is not sufficient to indicate annealing. However, when the morphological and mechanical data are taken together, the longer the duration of lysyl-oxidase treatment, the more integrated the interface appeared. Though further data are needed to confirm the mechanism of action, the enhancement of integration may be due to lysyl-oxidase-induced pyridinoline cross-links. This study demonstrates that lysyl-oxidase is a potent agent for enhancing integration between both native-to-native and native-to-engineered cartilages. The fact that interfacial strength increased manifold suggests that cross-linking agents should play a significant role in solving the difficult problem of cartilage integration. Future studies must examine dose, dosing regimen, and cellular responses to lysyl-oxidase to optimize its application.
登录
查看更多内容
影响因子：
4.1
作者：
Ionescu, Lara C.;Lee, Gregory C.;Mauck, Robert L.
通讯作者：
Mauck, Robert L.
影响因子：
0.8
作者：
Elder, Benjamin D.;Mohan, Arvind;Athanasiou, Kyriacos A.
通讯作者：
Athanasiou, Kyriacos A.
影响因子：
2.8
作者：
Obradovic, B;Martin, I;Vunjak-Novakovic, G
通讯作者：
Vunjak-Novakovic, G
影响因子：
2.8
作者：
Kon, Elizaveta;Delcogliano, Marco;Marcacci, Maurillo
通讯作者：
Marcacci, Maurillo
影响因子：
2.8
作者：
REINDEL, ES;AYROSO, AM;SAH, RL
通讯作者：
SAH, RL