Scale dependence of structure-function relationship in the emphysematous mouse lung.
Scale dependence of structure-function relationship in the emphysematous mouse lung.
复制标题
结构功能关系的比例依赖性在质量小鼠肺中。
DOI：
10.3389/fphys.2015.00146
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发表时间：
2015
影响因子：
4
通讯作者：
Suki B
中科院分区：
文献类型：
--
作者：
Sato S;Bartolák-Suki E;Parameswaran H;Hamakawa H;Suki B
本研究的目的是确定小鼠肺部弹性蛋白酶的初始分布如何决定组织破坏的时间过程以及不同空间尺度的结构异质性如何影响肺功能。我们评估了正常和肺气肿 C57BL/6 小鼠在用猪胰弹性蛋白酶 (PPE) 经口气管治疗后第 2 天和第 21 天的肺功能和肺泡结构。通过激光扫描共聚焦显微镜，使用红色荧光标记的 PPE (f-PPE) 评估治疗后 1 小时弹性蛋白酶的初始分布。根据测量的呼吸系统输入阻抗，可以获得整体肺顺应性和区域顺应性的变异性。固定肺并测量右肺四个肺叶和左肺三个区域的等效气腔直径。第2天和第21天，每个区域的平均空域直径显着扩大，并伴随着区域间异质性的增加。第 0 天的 f-PPE 沉积比第 2 天和第 21 天的区域间直径更加不均匀，并且在第 21 天达到统计显着性 (p < 0.05)。以空域直径总体变化为特征的微观尺度异质性与宏观尺度或区域间异质性相比，与依从性的相关性明显更好。此外，虽然炎症反应的空间分布似乎并不遵循弹性蛋白酶沉积的空间分布，但它与肺功能最强的区域决定因素相关。这些结果可能有助于解释人类肺气肿患者的肺功能下降（结构恶化）。
The purpose of this study was to determine how the initial distribution of elastase in mouse lungs determines the time course of tissue destruction and how structural heterogeneity at different spatial scales influences lung function. We evaluated lung function and alveolar structure in normal and emphysematous C57BL/6 mice at 2 and 21 days following orotracheal treatment with porcine pancreatic elastase (PPE). Initial distribution of elastase 1 h after treatment was assessed using red fluorescently labeled PPE (f-PPE) by laser scanning confocal microscopy. From measured input impedance of the respiratory system, the global lung compliance, and the variability of regional compliance were obtained. Lungs were fixed and equivalent airspace diameters were measured in four lobes of the right lung and three regions of the left lung. At day 2 and day 21, the mean airspace diameter of each region was significantly enlarged which was accompanied by an increased inter-regional heterogeneity. The deposition of f-PPE on day 0 was much more heterogeneous than the inter-regional diameters at both day 2 and day 21 and, at day 21, this reached statistical significance (p < 0.05). Microscale heterogeneity characterized by the overall variability of airspace diameters correlated significantly better with compliance than macroscale or inter-regional heterogeneity. Furthermore, while the spatial distribution of the inflammatory response does not seem to follow that of the elastase deposition, it correlates with the strongest regional determinant of lung function. These results may help interpret lung function decline in terms of structural deterioration in human patients with emphysema.
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影响因子：
5.8
作者：
Demedts, Ingel K.;Demoor, Tine;Bracke, Ken R.;Joos, Guy F.;Brusselle, Guy G.
通讯作者：
Brusselle, Guy G.
影响因子：
3.3
作者：
Emami, Kiarash;Cadman, Robert V.;Rizi, Rahim R.
通讯作者：
Rizi, Rahim R.
DOI：
10.1073/pnas.96.16.8829
发表时间：
1999-08-03
影响因子：
11.1
作者：
Mishima, M;Hirai, T;Suki, B
通讯作者：
Suki, B
影响因子：
3.3
作者：
Ito, S;Ingenito, EP;Suki, B
通讯作者：
Suki, B
影响因子：
6.9
作者：
O'Donnell, MD;O'Connor, CM;Martorana, PA
通讯作者：
Martorana, PA