Rapid flow-based peptide synthesis.
Rapid flow-based peptide synthesis.
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DOI：
10.1002/cbic.201300796
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发表时间：
2014-03-21
期刊：
影响因子：
3.2
通讯作者：
Pentelute, Bradley L.
中科院分区：
文献类型：
--
作者：
Simon, Mark D.;Heider, Patrick L.;Adamo, Andrea;Vinogradov, Alexander A.;Mong, Surin K.;Li, Xiyuan;Berger, Tatiana;Policarpo, Rocco L.;Zhang, Chi;Zou, Yekui;Liao, Xiaoli;Spokoyny, Alexander M.;Jensen, Klavs F.;Pentelute, Bradley L.
描述了一种基于流动的固相肽合成方法，其能够在自动控制下每1.8分钟或在手动控制下每3分钟掺入一个氨基酸残基。这是通过使试剂流通过热交换器进入低体积、低背压反应容器并通过UV检测器来实现的。这些特征使得能够以高流速将加热的溶剂和试剂连续输送到固体支持物，保持反应容器中试剂的最大浓度，快速交换试剂，并消除在将试剂添加到容器后快速加热试剂的需要。UV检测器可对过程进行连续监控。为了证明这种方法的广泛适用性和可靠性，它被用于一种小蛋白质以及几十种肽的全合成。用该方法获得的材料的质量与传统的分批方法相当，并且在所有情况下，所需的材料易于通过RP-HPLC纯化。并将此方法应用于113位残基B的合成。在所附手稿中描述了解淀粉芽孢杆菌RNA酶和130个残基的pE 59 DARPin。
A flow-based solid phase peptide synthesis methodology that enables the incorporation of an amino acid residue every 1.8 minutes under automatic control, or every three minutes under manual control, is described. This is accomplished by passing a stream of reagent through a heat exchanger, into a low volume, low backpressure reaction vessel, and through a UV detector. These features enable the continuous delivery of heated solvents and reagents to the solid support at high flow rate, maintaining a maximal concentration of reagents in the reaction vessel, quickly exchanging reagents, and eliminating the need to rapidly heat reagents after they have been added to the vessel. The UV detector enables continuous monitoring of the process. To demonstrate the broad applicability and reliability of this method, it was employed in the total synthesis of a small protein, as well as dozens of peptides. The quality of the material obtained with this method is comparable to traditional batch methods, and, in all cases, the desired material was readily purifiable via RP-HPLC. The application of this method to the synthesis of the 113 residue B. amyloliquefaciens RNase and the 130 residue pE59 DARPin is described in the accompanying manuscript.
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影响因子：
3.6
作者：
Han, YX;Albericio, F;Barany, G
通讯作者：
Barany, G
影响因子：
15
作者：
Pentelute BL;Gates ZP;Tereshko V;Dashnau JL;Vanderkooi JM;Kossiakoff AA;Kent SB
通讯作者：
Kent SB
影响因子：
56.9
作者：
DAWSON, PE;MUIR, TW;KENT, SBH
通讯作者：
KENT, SBH
DOI：
10.1073/pnas.96.4.1181
发表时间：
1999-02-16
影响因子：
11.1
作者：
Miranda, LP;Alewood, PF
通讯作者：
Alewood, PF
影响因子：
2.1
作者：
Souza, MP;Tavares, MFM;Miranda, MTM
通讯作者：
Miranda, MTM