Far-red light photoactivatable near-infrared fluorescent proteins engineered from a bacterial phytochrome.
Far-red light photoactivatable near-infrared fluorescent proteins engineered from a bacterial phytochrome.
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DOI：
10.1038/ncomms3153
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发表时间：
2013
影响因子：
16.6
通讯作者：
Verkhusha, Vladislav V.
中科院分区：
文献类型：
--
作者：
Piatkevich, Kiryl D.;Subach, Fedor V.;Verkhusha, Vladislav V.
光学探针的荧光调制能力可用于提高在高度自发荧光的环境中成像的信噪比，例如完整的组织和活的生物体。在这里，我们报道了两种基于光敏色素的可光激活的近红外荧光蛋白，命名为PAiRFP1和PAiRFP2。PAiRFPs利用血红素衍生的胆绿素作为发色团，这种胆绿素广泛存在于哺乳动物组织中。经过短暂的远红光照射后，最初弱荧光的PAiRFP被光转化为高荧光状态，激发/发射波长为690 nm/717 nm。在光激活后，PAiRFPs慢慢恢复到初始状态，从而实现多个光激活-松弛循环。低温光谱分析揭示了参与PAiRFP光循环的几个中间体，它们都不同于细菌光敏色素前体的中间体。PAiRFPs可以在小鼠组织中以空间选择性的方式被光激活，其荧光的光学调制允许显著的对比度增强，使得PAiRFPs在高自然荧光和低信号水平的活体成像条件下比永久荧光探针更具优势。
Ability to modulate fluorescence of optical probes can be used to enhance signal-to-noise ratio for imaging within highly autofluorescent environments, such as intact tissues and living organisms. Here we report two phytochrome-based photoactivatable near-infrared fluorescent proteins, named PAiRFP1 and PAiRFP2. PAiRFPs utilize heme-derived biliverdin, ubiquitous in mammalian tissues, as the chromophore. Initially weakly fluorescent PAiRFPs undergo photoconversion into a highly fluorescent state with excitation/emission at 690 nm/717 nm following a brief irradiation with far-red light. After photoactivation, PAiRFPs slowly revert back to initial state, enabling multiple photoactivation-relaxation cycles. Low-temperature optical spectroscopy reveals several intermediates involved in PAiRFP photocycles, which all differ from that of the bacteriophytochrome precursor. PAiRFPs can be photoactivated in a spatially selective manner in mouse tissues, and optical modulation of their fluorescence allows for substantial contrast enhancement, making PAiRFPs advantageous over permanently fluorescent probes for in vivo imaging conditions of high autofluorescence and low signal levels.
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DOI：
10.1126/science.1168683
发表时间：
2009-05-08
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Shu X;Royant A;Lin MZ;Aguilera TA;Lev-Ram V;Steinbach PA;Tsien RY
通讯作者：
Tsien RY
DOI：
10.1016/j.jphotobiol.2008.07.001
发表时间：
2008-10-16
影响因子：
5.4
作者：
Krieger, Alexander;Molina, Isabel;Lamparter, Tilman
通讯作者：
Lamparter, Tilman
遗传编码的近红外荧光探针的深组织光声断层扫描。
影响因子：
16.6
作者：
Filonov, Grigory S.;Krumholz, Arie;Xia, Jun;Yao, Junjie;Wang, Lihong V.;Verkhusha, Vladislav V.
通讯作者：
Verkhusha, Vladislav V.
影响因子：
4.8
作者：
Borucki, B;von Stetten, D;Hildebrandt, P
通讯作者：
Hildebrandt, P
影响因子：
3.2
作者：
Rottwinkel, Gregor;Oberpichler, Inga;Lamparter, Tilman
通讯作者：
Lamparter, Tilman