The unusual action of (R,S)-2-hydroxy-2-trifluoromethyl-trans-n-octadec-4-enoic acid on 5-lipoxygenase from potato tubers.
The unusual action of (R,S)-2-hydroxy-2-trifluoromethyl-trans-n-octadec-4-enoic acid on 5-lipoxygenase from potato tubers.
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(R,S)-2-羟基-2-三氟甲基-反式-正十八-4-烯酸对马铃薯块茎中 5-脂氧合酶的不寻常作用。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
V. Kukhar
中科院分区：
文献类型：
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作者：
Igor A. Butovich;V. Soloshonok;V. Kukhar
我们发现，(R，S)-2-hydroxy-2-trifluoromethyl-trans-n-octadec-4-enoic酸)对马铃薯块茎中的5-脂氧合酶有很强的激活作用。酶的活化度与HTFOA浓度成正比，在约0.1 mM时达到最大值，与初始底物浓度(25微米或0.1 mM)无关。当HTFOA的浓度较高时，就会发生酶抑制。酶的活性与底物(亚油酸)浓度成反比。这一结果可以通过假设酶分子中存在一个调节中心来解释，该中心对激活剂和亚油酸这两种物质都显示出亲和力。
We found that (R,S)-2-hydroxy-2-trifluoromethyl-trans-n-octadec-4-enoic acid (HTFOA) is a powerful activator of 5-lipoxygenase from potato tubers. The degree of activation of the enzyme is proportional to the HTFOA concentration and is a maximum at about 0.1 mM independently of initial substrate concentration (25 microM or 0.1 mM). At greater concentrations of HTFOA, enzyme inhibition takes place. Enzyme activation is inversely proportional to the substrate (linoleic acid) concentration. The results may be explained by assuming that a regulatory center exists in the enzyme molecule, which shows affinity to both substances: activator and linoleic acid.
使用 Swinbourne 图研究潜在的自杀底物：ATP 和 ADP 对酵母线粒体 F1-ATP 酶的影响。
影响因子：
2.9
作者：
Jenkins,WT
通讯作者：
Jenkins,WT