Helicobacter ganmani sp. nov., a urease-negative anaerobe isolated from the intestines of laboratory mice.
Helicobacter ganmani sp. nov., a urease-negative anaerobe isolated from the intestines of laboratory mice.
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甘马螺杆菌 sp.
DOI：
--
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发表时间：
2001
影响因子：
2.8
通讯作者：
Adrian K. C. Lee
中科院分区：
文献类型：
--
作者：
B. Robertson;J. O'Rourke;P. Vandamme;S. On;Adrian K. C. Lee
在一项研究中，从实验室小鼠的肠道中分离出螺旋细菌。该研究检查了在澳大利亚悉尼四个不同动物设施中饲养的小鼠是否存在螺旋杆菌物种和其他螺旋生物。从四个设施中的三个设施的小鼠身上培养出来的17个菌株中，有一组被发现是螺旋杆菌，但不属于目前公认的18个物种中的任何一个。这些菌株的不同寻常之处在于，它们只能在37摄氏度的温度下厌氧生长，并且不能在微氧条件下生长。与轮状幽门螺杆菌一样，分离株有单极、双极、无鞘的鞭毛，尿素酶阴性。它们对氧化酶呈阳性，能将硝酸盐还原为亚硝酸盐，但不能水解马尿酸盐或乙酸吲哚，生长在木炭琼脂上，对头孢菌素具有抗药性。测定了4个菌株的16S rDNA序列，发现它们是完全相同的。在16S rDNA序列相似性方面，罗齿藻的同源性最高(98.2%)。用SDS-PAGE对9个菌株的全细胞蛋白质进行了数值分析，并与所有已知的螺杆菌种进行了比较，其中包括新确定的3个轮状幽门螺杆菌菌株的蛋白质谱。新分离株与轮状幽门螺杆菌和其他幽门螺杆菌有明显区别。根据这些数据，包括遗传、生化和蛋白质分析，这些分离物被认为属于甘肃螺杆菌。11月(模式菌株CMRI H02T=CCUG 43526T=CIP 106846T)。
Spiral bacteria were isolated from the intestines of laboratory mice during a study examining the presence of Helicobacter species and other spiral organisms naturally infecting mice maintained at four different animal facilities in Sydney, Australia. One group of 17 isolates, cultured from mice from three of the four facilities, were found to be helicobacters but did not fall within any of the 18 currently recognized species. These isolates were unusual in that they only grew anaerobically at 37 degrees C and were incapable of growth under microaerobic conditions. Like Helicobacter rodentium, isolates possessed single, bipolar, unsheathed flagella and were urease-negative. They were positive for oxidase and reduced nitrate to nitrite but did not hydrolyse hippurate or indoxyl acetate, grew on charcoal agar and were resistant to cephalothin. 16S rDNA sequences from four strains were determined and found to be identical to one another. H. rodentium was the most closely related species in terms of 16S rDNA sequence similarity (98.2%). Numerical analysis of whole-cell proteins by SDS-PAGE for nine isolates was carried out with a comparison to all known Helicobacter species, including newly determined profiles from three H. rodentium strains. The new isolates were clearly differentiated from H. rodentium and other Helicobacter spp. On the basis of this data, including genetic, biochemical and protein analysis, it is proposed that these isolates belong to Helicobacter ganmani sp. nov. (type strain CMRI H02T = CCUG 43526T = CIP 106846T).
DOI：
10.1099/00207713-47-3-627
发表时间：
1997-07
期刊：
International journal of systematic bacteriology
影响因子：
--
作者：
Zeli Shen;James G. Fox;F. Dewhirst;B. J. Paster;C. Foltz;L. Yan;Brian D. Shames;L. Perry
通讯作者：
Zeli Shen;James G. Fox;F. Dewhirst;B. J. Paster;C. Foltz;L. Yan;Brian D. Shames;L. Perry
DOI：
10.1099/00207713-46-4-916
发表时间：
1996
期刊：
International journal of systematic bacteriology.
影响因子：
--
作者：
Mendes,EN;Queiroz,DM;Dewhirst,FE;Paster,BJ;Moura,SB;Fox,JG
通讯作者：
Fox,JG
螺杆菌和肝脏疾病。
DOI：
--
发表时间：
1997
期刊：
Italian journal of gastroenterology and hepatology
影响因子：
--
作者：
Fox,JG;Wang,TC
通讯作者：
Wang,TC