γ/δ T cell antigen receptors expressed on tumor‐infiltrating lymphocytes from patients with solid tumors-猫眼课题宝

实体瘤患者肿瘤浸润淋巴细胞上表达的 γ/δ T 细胞抗原受体

DOI：

发表时间：

1992

期刊：

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

M. Nanno;H. Seki;George Mathioudaki;R. Suzuki;K. Itoh;C. Ioannides;S. Suzuki;Pei;C. Platsoucas

The expression of γ/δ T cell antigen receptors (TcR) in T cell lines or clones derived from tumor‐infiltrating lymphocytes (TIL) from patients with solid tumors was investigated. γ/δ TcR T cell lines were derived from TIL from patients with Wilms tumor, sarcoma or metastatic melanoma by stimulation with autologous tumor cells alone and recombinant interleukin 2 and they exhibited nonspecific cytotoxicity against autologous and allogeneic tumor cells, or cells of the K562 or the MEL21 tumor cell lines. Two T cell lines were derived from a patient with Wilms tumor. One of them expressed a non‐disulfide‐linked γ/δ TcR using the 60‐kDa γ chain, whereas, the other expressed a disulfide‐linked γ/δ TcR. A T cell line was derived from a patient with sarcoma and expressed a disulfide‐linked γ/δ TcR, whereas, a T cell line derived from a patient with melanoma expressed a non‐disulfide‐linked γ chain of 62 kDa. Several T cell clones were developed from patients with metastatic melanoma or Wilms tumor and expressed either disulfide‐ or non‐disulfide‐linked γ/δ TcR. Northern analysis of RNA from certain of these clones revealed a full‐length γ chain transcript, whereas, the α or β chain transcripts were either absent or truncated. These T cell clones exhibited nonspecific cytotoxicity. Both disulfide‐ and non‐disulfide‐linked TIL T cell lines and clones expressed the δ TCS1 determinant. γ/δ TcR+ cells in freshly prepared TIL from these patients were present in low proportions (<5%) and their δTCS1/δ1 ratios were within the range observed in the peripheral blood of normal donors. These results demonstrate that both disulfide‐and non‐disulfide‐linked γ/δ TcR are expressed on T cell lines and clones derived from TIL from solid tumors. Non‐disulfide‐linked γ/δ TcR using the 56–66‐kDa γ chain are frequently found on TIL‐derived T cell lines and clones. These 56–66‐kDa γ chains are rarely expressed on T cell lines or clones derived from peripheral blood lymphocytes of normal donors.