Unique and redundant functions of cytoplasmic actins and nonmuscle myosin II isoforms at epithelial junctions.
Unique and redundant functions of cytoplasmic actins and nonmuscle myosin II isoforms at epithelial junctions.
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DOI：
10.1111/nyas.14808
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发表时间：
2022-09
影响因子：
5.2
通讯作者：
Naydenov NG
中科院分区：
文献类型：
--
作者：
Ivanov AI;Lechuga S;Marino-Melendez A;Naydenov NG
上皮屏障的完整性和功能依赖于粘附连接（AJs）和紧密连接（TJs）的形成。AJs和TJs的一个特征是它们与由肌动蛋白丝和非肌肉肌球蛋白II（NM-II）马达组成的皮质细胞骨架相关联。肌动球蛋白细胞骨架产生的机械力对于连接装配、稳定性和重塑是必不可少的。上皮细胞表达两种不同的肌动蛋白和三种NM-II亚型，所有这些都与AJs和TJs相关。尽管它们的结构相似，但不同的肌动蛋白和NM-II亚型具有不同的生化特性，细胞分布和功能。上皮肌动蛋白和肌球蛋白马达的多样性可能是至关重要的连接的形成，成熟和拆卸的不同步骤的调节。本文综述了肌动蛋白和NM-II亚型在控制各种上皮细胞的完整性和屏障特性中的作用。我们讨论了个体肌动蛋白亚型和NM-II马达的耗竭对体外模型上皮单层中AJs和TJ的组装和屏障功能的影响。我们还描述了不同肌动蛋白和NM-II马达的全部或组织特异性基因敲除的功能后果，重点是体内不同上皮细胞的发育和完整性。上皮屏障的完整性和功能由细胞间连接和皮质肌动球蛋白细胞骨架之间的相互作用调节。连接相关的细胞骨架由不同的肌动蛋白和非肌肉肌球蛋白II亚型构建。在这里，我们概述了不同的细胞质肌动蛋白和肌球蛋白II电机与上皮粘附和紧密连接的相互作用，以及这些细胞骨架蛋白在调节上皮屏障的完整性和重塑的功能作用。
The integrity and functions of epithelial barriers depend on the formation of adherens junctions (AJs) and tight junctions (TJs). A characteristic feature of AJs and TJs is their association with the cortical cytoskeleton composed of actin filaments and nonmuscle myosin II (NM‐II) motors. Mechanical forces generated by the actomyosin cytoskeleton are essential for junctional assembly, stability, and remodeling. Epithelial cells express two different actin proteins and three NM‐II isoforms, all known to be associated with AJs and TJs. Despite their structural similarity, different actin and NM‐II isoforms have distinct biochemical properties, cellular distribution, and functions. The diversity of epithelial actins and myosin motors could be essential for the regulation of different steps of junctional formation, maturation, and disassembly. This review focuses on the roles of actin and NM‐II isoforms in controlling the integrity and barrier properties of various epithelia. We discuss the effects of the depletion of individual actin isoforms and NM‐II motors on the assembly and barrier function of AJs and TJs in model epithelial monolayers in vitro. We also describe the functional consequences of either total or tissue‐specific gene knockout of different actins and NM‐II motors, with a focus on the development and integrity of different epithelia in vivo. The integrity and functions of epithelial barriers is regulated by the cross‐talk between intercellular junctions and the cortical actomyosin cytoskeleton. The junction‐associated cytoskeleton is built by different actin and non‐muscle myosin II isoforms. Here we overview the interactions of different cytoplasmic actins and myosin II motors with epithelial adherens and tight junctions and functional roles of these cytoskeletal proteins in regulating epithelial barrier integrity and remodeling.
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影响因子：
16.6
作者：
Chen A;Arora PD;McCulloch CA;Wilde A
通讯作者：
Wilde A
影响因子：
64.8
作者：
Cavey, Matthieu;Rauzi, Matteo;Lecuit, Thomas
通讯作者：
Lecuit, Thomas
DOI：
10.1073/pnas.0900221106
发表时间：
2009-06-16
影响因子：
11.1
作者：
Belyantseva, Inna A.;Perrin, Benjamin J.;Ervasti, James M.
通讯作者：
Ervasti, James M.
影响因子：
--
作者：
Kim JS;Lee GJ;Lee TW;Ihm CG;Lee YH;Kim YG;Moon JY;Lee SH;Kim JH;Kang SW;Chae SJ;Park HK;Jeong KH
通讯作者：
Jeong KH
影响因子：
7.2
作者：
Anderson, James M.;Van Itallie, Christina M.
通讯作者：
Van Itallie, Christina M.