Observations of the Effects of Angiotensin II Receptor Blocker on Angiotensin II-Induced Morphological and Mechanical Changes in Renal Tubular Epithelial Cells Using Atomic Force Microscopy.
Observations of the Effects of Angiotensin II Receptor Blocker on Angiotensin II-Induced Morphological and Mechanical Changes in Renal Tubular Epithelial Cells Using Atomic Force Microscopy.
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DOI：
10.1155/2018/9208795
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发表时间：
2018
影响因子：
--
通讯作者：
Jeong KH
中科院分区：
文献类型：
--
作者：
Kim JS;Lee GJ;Lee TW;Ihm CG;Lee YH;Kim YG;Moon JY;Lee SH;Kim JH;Kang SW;Chae SJ;Park HK;Jeong KH
血管紧张素 II (Ang II) 在肾脏中发挥促纤维化作用。尽管Ang II的许多途径已被发现，但其形态和力学方面尚未得到很好的研究。我们使用原子力显微镜 (AFM) 观察了使用或不使用 Ang II 受体阻滞剂 (ARB) 的 Ang II 治疗后肾小管上皮细胞 (TEC) 的变化。 用 Ang II 联合或不联合替米沙坦、PD123319 和 blebbistatin 刺激 TEC。进行 AFM 来测量细胞硬度、细胞体积和细胞表面粗糙度。通过免疫细胞化学测定上皮到间质的转变标记。 Ang II 刺激后，细胞转化为扁平且拉长的间质形态。 Ang II 处理的 TEC 中细胞表面粗糙度和体积显着增加。 Ang II 还诱导磷酸肌球蛋白轻链和 F-肌动蛋白的增加以及 E-钙粘蛋白的减少。 Ang II 与替米沙坦或布雷他汀联合孵育可减弱 Ang II 诱导的这些变化。 我们首次报告使用 AFM 直接观察 Ang II 治疗（有或没有 ARB）后 TEC 的变化。同时，我们成功测量了PD123319或blebbistatin的选择性作用。 AFM 可能是 TEC 细胞过程的一种非侵入性评估策略。
Angiotensin II (Ang II) plays a profibrotic role in the kidneys. Although many pathways of Ang II have been discovered, the morphological and mechanical aspects have not been well investigated. We observed the changes in tubular epithelial cells (TECs) after Ang II treatment with or without Ang II receptor blockers (ARBs) using atomic force microscopy (AFM). TECs were stimulated with Ang II with or without telmisartan, PD123319, and blebbistatin. AFM was performed to measure the cellular stiffness, cell volume, and cell surface roughness. Epithelial to mesenchymal transition markers were determined via immunocytochemistry. After Ang II stimulation, cells transformed to a flattened and elongated mesenchymal morphology. Cell surface roughness and volume significantly increased in Ang II treated TECs. Ang II also induced an increase in phospho-myosin light chain and F-actin and a decrease in E-cadherin. Ang II coincubation with either telmisartan or blebbistatin attenuated these Ang II-induced changes. We report, for the first time, the use of AFM in directly observing the changes in TECs after Ang II treatment with or without ARBs. Simultaneously, we successfully measured the selective effect of PD123319 or blebbistatin. AFM could be a noninvasive evaluating strategy for cellular processes in TECs.
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影响因子：
5.3
作者：
Chen, Jianchun;Chen, Jian-Kang;Harris, Raymond C.
通讯作者：
Harris, Raymond C.
影响因子：
2.8
作者：
Jeong, Kyung-Hwan;Lee, Tae-Won;Lee, Sang-Ho
通讯作者：
Lee, Sang-Ho
影响因子：
9.9
作者：
Rabinovich, Y;Esayanur, M;Khan, S
通讯作者：
Khan, S
影响因子：
0.8
作者：
Sharma, Shivani;Gimzewski, James K.
通讯作者：
Gimzewski, James K.
影响因子：
3.4
作者：
Gad, M;Ikai, A
通讯作者：
Ikai, A