Silencing Nicotiana attenuata LHY and ZTL alters circadian rhythms in flowers.
Silencing Nicotiana attenuata LHY and ZTL alters circadian rhythms in flowers.
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DOI：
10.1111/nph.13681
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发表时间：
2016-02
期刊：
影响因子：
--
通讯作者：
Kim SG
中科院分区：
文献类型：
--
作者：
Yon F;Joo Y;Cortés Llorca L;Rothe E;Baldwin IT;Kim SG
花的有节奏的开/关和挥发性物质的释放在特定的时间吸引传粉者。这些节律在恒定的光照或黑暗条件下保持，表明生物钟参与其中。虽然正向和反向遗传方法导致了拟南芥中核心昼夜节律钟组件的鉴定，但这些时钟组件对花节律的参与仍未得到验证，这可能是由于A.拟南芥花。在这里，我们解决了这些核心时钟组件在野生烟草的花中的作用，其花在夜间开放，释放苄基丙酮（BA）气味，并垂直移动通过140°弧。我们首先测量了N。attenuata花的节奏在恒定的光照条件下。结果表明，昼夜节律钟控制花开放，BA排放，花梗运动，但不关闭。我们获得了转基因N.在拟南芥晚伸长下胚轴（LHY）和ZEITLUPE（ZTL）的同源基因中沉默的渐狭拟南芥品系中，所述同源基因被称为核心时钟组分。沉默NaLHY和NaZTL以不同的方式强烈地改变了花的节律，表明N. attenuata协调这些花的节奏。
The rhythmic opening/closing and volatile emissions of flowers is known to attract pollinators at specific times. That these rhythms are maintained under constant light or dark conditions suggests a circadian clock involvement. Although a forward and reverse genetic approach led to the identification of core circadian clock components in Arabidopsis thaliana, involvement of these clock components for floral rhythms remained untested likely due to weak diurnal rhythms in A. thaliana flowers. Here we addressed the role of these core clock components in the flowers of the wild tobacco Nicotiana attenuata, whose flowers open at night, emit benzyl acetone (BA) scents, and move vertically through a 140° arc. We first measured N. attenuata floral rhythms under constant light conditions. The results suggest that the circadian clock controls flower opening, BA emission, and pedicel movement, but not flower closing. We generated transgenic N. attenuata lines silenced in the homologous genes of Arabidopsis LATE ELONGATED HYPOCOTYL (LHY) and ZEITLUPE (ZTL), which are known as a core clock component. Silencing NaLHY and NaZTL strongly altered floral rhythms in different ways, indicating that conserved clock components in N. attenuata coordinate these floral rhythms.
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影响因子：
56.9
作者：
Huang, W.;Perez-Garcia, P.;Mas, P.
通讯作者：
Mas, P.
影响因子：
64.8
作者：
Más, P;Kim, WY;Kay, SA
通讯作者：
Kay, SA
影响因子：
64.8
作者：
Endo, Motomu;Shimizu, Hanako;Nohales, Maria A.;Araki, Takashi;Kay, Steve A.
通讯作者：
Kay, Steve A.
DOI：
10.1073/pnas.1422875112
发表时间：
2015-08-04
影响因子：
11.1
作者：
Fenske, Myles P.;Hazelton, Kristen D. Hewett;Imaizumi, Takato
通讯作者：
Imaizumi, Takato
影响因子：
7.2
作者：
Bhattacharya, Samik;Baldwin, Ian T.
通讯作者：
Baldwin, Ian T.