Investigating the role of Aurora kinases in RAS signaling.
Investigating the role of Aurora kinases in RAS signaling.
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研究了极光激酶在RAS信号传导中的作用。
DOI：
10.1002/jcb.21974
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发表时间：
2009-01-01
影响因子：
4
通讯作者：
中科院分区：
文献类型：
--
作者：
Activating ras mutations are frequently found in malignant tumors of the pancreas, colon, lung and other tissues. RAS activates a number of downstream pathways that ultimately cause cellular transformation. Several recent studies suggested that one of those pathways involves Aurora kinases. Overexpression of Aurora-B kinase can augment transformation by oncogenic RAS, however the mechanism was not determined. The cooperative effect of high levels of Aurora kinase is important since this kinase is frequently overexpressed in human tumors. We have used two Aurora kinase inhibitors to test effect on RAS signaling. We find that these inhibitors have no effect on the phosphorylation of MEK1/2 or MAPK in response to RAS. Furthermore, inhibiting Aurora kinases in human cancer cells with or without activated RAS did not change the length of the cell cycle nor induce apoptosis suggesting that these kinases do not play a direct role in these key cellular responses to activated RAS. Overexpression of Aurora B can cause cells to become polyploid. Also, inducing polyploidy with cytochalasin D was reported to induce neoplastic transformation, suggesting that Aurora overexpression may cooperate with RAS indirectly by inducing polyploidy. We find that inducing polyploidy with cytochalasin D or blebbistatin does not enhance transformation by oncogenic RAS. Our observations argue against a direct role for Aurora kinases in the RAS-MAPK pathway, and suggest that the polyploid state does not enhance transformation by RAS.
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影响因子：
8
作者：
Stiegler, P;Schuchner, S;Wintersberger, E
通讯作者：
Wintersberger, E
影响因子：
3.3
作者：
Taylor, WR;DePrimo, SE;Stark, GR
通讯作者：
Stark, GR
影响因子：
5.8
作者：
Sorrentino, R;Libertini, S;Portella, G
通讯作者：
Portella, G
小分子黄质蛋白揭示了Aurora B在校正动型微管附着和维持纺锤体组装检查点方面的作用。
DOI：
10.1083/jcb.200208092
发表时间：
2003-04-28
期刊：
The Journal of cell biology
影响因子：
--
作者：
Hauf S;Cole RW;LaTerra S;Zimmer C;Schnapp G;Walter R;Heckel A;van Meel J;Rieder CL;Peters JM
通讯作者：
Peters JM
影响因子：
11.4
作者：
BROWN, R;MARSHALL, CJ;HALL, A
通讯作者：
HALL, A