Stability and structure of DNA oligonucleotides containing non-specific base analogues.
Stability and structure of DNA oligonucleotides containing non-specific base analogues.
复制标题
含有非特异性碱基类似物的 DNA 寡核苷酸的稳定性和结构。
DOI：
10.1006/jmbi.1997.1129
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发表时间：
1997
影响因子：
5.6
通讯作者：
S. Salisbury
中科院分区：
文献类型：
--
作者：
D. Loakes;F. Hill;D. M. Brown;S. Salisbury
研究了含5-硝基吲哚脱氧核糖基衍生物的DNA的性质。5-硝基吲哚已被证明在与天然碱基相对的双链体中存在时具有良好的稳定性，仅具有略微降低的解链温度。然而，当它作为额外的凸出碱基掺入双链体中时，稳定性增强。当其作为悬垂碱基存在于两条链的5'或3'-末端时，其还显著增强双链体的稳定性。的稳定被推测是由于增强堆积相互作用的硝基吲哚基地。含有许多连续的5-硝基吲哚残基的低聚物形成稳定的堆叠二级结构。含有21个这样的取代的低聚物被认为是作为发夹结构存在的。这是进一步研究的圆二色性熔解实验，这表明，单链寡聚体含有显着的二级结构的区域中的5-硝基吲哚道，这似乎包含一个双链茎。5-硝基吲哚脱氧核苷的X射线分析提供了一些指示，说明在核苷晶体中观察到的堆积模式也可能是稳定寡核苷酸二级结构的原因。
The nature of DNA containing the deoxyribosyl derivative of 5-nitroindole has been investigated. 5-Nitroindole has been shown to give good stability when present in duplexes opposite natural bases, with only slightly reduced melting temperatures. However, enhanced stability occurs when it is incorporated as an additional bulged base in duplexes. It also markedly enhances the stability of duplexes when it is present as a pendant base at either the 5' or 3'-ends of the two strands. The stabilisation is presumed to be due to enhanced stacking interactions for the nitroindole base. Oligomers containing a number of consecutive 5-nitroindole residues form stable, stacked secondary structures. An oligomer containing 21 such substitutions is presumed to exist as a hairpin structure. This was further investigated by circular dichroism melting experiments, which demonstrated that the single-stranded oligomer contains significant secondary structure in the region of the 5-nitroindole tract, which appears to contain a double-stranded stem. X-ray analysis of 5-nitroindole deoxyriboside provides some indication of how the mode of stacking observed in crystals of the nucleoside may also be responsible for stabilising secondary structures of oligonucleotides.
影响因子：
14.9
作者：
MARTIN, FH;CASTRO, MM;TINOCO, I
通讯作者：
TINOCO, I
通过 1H NMR 确定 G.C 区域内凸出的鸟苷的优先位置。
影响因子：
2.9
作者：
Woodson,SA;Crothers,DM
通讯作者：
Crothers,DM
影响因子：
15
作者：
Guckian, KM;Schweitzer, BA;Kool, ET
通讯作者：
Kool, ET