Functional diversity of vertebrate ARNT proteins: identification of ARNT2 as the predominant form of ARNT in the marine teleost, Fundulus heteroclitus.
Functional diversity of vertebrate ARNT proteins: identification of ARNT2 as the predominant form of ARNT in the marine teleost, Fundulus heteroclitus.
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脊椎动物 ARNT 蛋白的功能多样性：将 ARNT2 鉴定为海洋硬骨鱼、异斜眼底 ARNT 的主要形式。
DOI：
10.1006/abbi.1998.0992
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发表时间：
1999
影响因子：
3.9
通讯作者：
Hahn,ME
中科院分区：
文献类型：
--
作者：
Powell,WH;Karchner,SI;Bright,R;Hahn,ME
芳香烃受体核转运蛋白（ARNT）是bHLH/PAS蛋白超家族的成员。ARNT与几个PAS超家族成员（包括配体激活的芳烃受体（AHR））二聚化，形成一种复合物，通过结合靶基因启动子内的特异性元件来改变转录。啮齿动物中有两种基因编码不同形式的蛋白质：广泛表达的ARNT 1和ARNT 2，ARNT 2仅限于成人的大脑和肾脏以及胚胎的特定神经和鳃组织。在努力表征芳香烃信号机制inFundulus heteroclitus，海洋硬骨鱼，可以开发遗传性异生物质抗性，我们已经分离出肝脏cDNA编码的ARNT同源物。该蛋白质表现出其他脊椎动物ARNT典型的AHR依赖性DNA结合能力。出乎意料的是，系统发育分析显示，该cDNA编码ARNT 2。这是唯一可检测到的ARNT序列在底肝，鳃，卵巢，和脑，这表明ARNT 2是ARNT的主要形式在这个物种。同样令人惊讶的是，相对缺乏序列同一性的另一种鱼ARNT蛋白，虹鳟鱼ARNTb，我们显示形成一个独特的分支以外的ARNT 1和ARNT 2的进化枝在系统发育分析。鱼类ARNT蛋白的功能多样性可能对评估芳烃对自然种群的影响具有重要意义。越来越多地使用鱼类模型在发展和毒理学研究强调了识别分类特异性作用的ARNT蛋白及其潜在的二聚体伙伴在PAS超家族的重要性。
The aryl hydrocarbon receptor nuclear translocator (ARNT) is a member of the bHLH/PAS protein superfamily. ARNT dimerizes with several PAS superfamily members, including the ligand-activated aryl hydrocarbon receptor (AHR), forming a complex that alters transcription by binding specific elements within the promoters of target genes. Two genes encode different forms of the protein in rodents: ARNT1, which is widely expressed, and ARNT2, which is limited to the brain and kidneys of adults and specific neural and branchial tissues of embryos. In an effort to characterize aryl hydrocarbon signaling mechanisms inFundulus heteroclitus,a marine teleost that can develop heritable xenobiotic resistance, we have isolated a liver cDNA encoding an ARNT homolog. The protein exhibits AHR-dependent DNA binding capability typical of other vertebrate ARNTs. Unexpectedly, phylogenetic analysis reveals that the cDNA encodes an ARNT2. This is the only detectable ARNT sequence inFundulusliver, gill, ovary, and brain, suggesting that ARNT2 is the predominant form of ARNT in this species. Also surprising is the relative lack of sequence identity with another fish ARNT protein, rainbow trout ARNTb, which we show forms a distinct branch outside the ARNT1 and ARNT2 clades in phylogenetic analyses. Functional diversity of ARNT proteins in fish may have important implications for the assessment of aryl hydrocarbon effects on natural populations. The increasing use of fish models in developmental and toxicological studies underscores the importance of identifying taxon-specific roles of ARNT proteins and their potential dimeric partners in the PAS superfamily.
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影响因子：
56.9
作者：
Crosthwaite, SK;Dunlap, JC;Loros, JJ
通讯作者：
Loros, JJ
影响因子：
2.7
作者：
Kozak, KR;Abbott, B;Hankinson, O
通讯作者：
Hankinson, O
污染环境中的耐受力和压力木乃伊猪的案例
DOI：
--
发表时间：
1989
期刊：
影响因子：
--
作者：
J. Weis;P. Weis
通讯作者：
P. Weis
影响因子：
14.9
作者：
CARVER, LA;HOGENESCH, JB;BRADFIELD, CA
通讯作者：
BRADFIELD, CA
影响因子：
14.9
作者：
THOMPSON, JD;HIGGINS, DG;GIBSON, TJ
通讯作者：
GIBSON, TJ