Low-cost 3D-printed inverted microscope to detect Mycobacterium tuberculosis in a MODS culture.
Low-cost 3D-printed inverted microscope to detect Mycobacterium tuberculosis in a MODS culture.
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DOI：
10.1016/j.tube.2021.102158
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发表时间：
2022-01
期刊：
影响因子：
--
通讯作者：
Zimic M
中科院分区：
文献类型：
--
作者：
Salguedo M;Zarate G;Coronel J;Comina G;Gilman RH;Sheen P;Oberhelman R;Zimic M
MODS是一种诊断结核病和药物敏感性的检测方法，它基于对液体培养基中生长的结核分枝杆菌菌落特征索的显微镜观察。需要倒置光学显微镜（100倍放大）观察和解释MODS培养。不幸的是，在低资源环境下，商业倒置显微镜的成本是负担不起的。使用MODS检测进行结核病诊断时，质量适中的图像足以进行适当的解释。因此，使用高成本的商用倒置光学显微镜并不是必不可少的。在这项研究中，我们设计了一个基于智能手机的3d打印光学倒置显微镜的原型。采用226张MODS结核阳性图像和207张MODS结核阴性图像对系统进行评价。这些图像分别来自10份MODS阳性和10份MODS阴性的痰样本。所有图像的质量由合格的技术人员进行评估，以充分解释并将其分类为结核阳性或阴性。图像质量被认为适合MODS解译。通过3d打印倒置显微镜的原型读取，将20个样本正确分类（结核阳性/阴性）。
MODS, an assay for diagnosis of tuberculosis and drug-susceptibility, is based in the microscopic observation of the characteristic cords of Mycobacterium tuberculosis colonies grown in liquid media. An inverted optical microscope (100X magnification) is required to observe and interpret MODS cultures. Unfortunately, the cost of commercial inverted microscopes is not affordable in low resource settings. To perform a diagnosis of tuberculosis using the MODS assay, images with modest quality are enough for proper interpretation. Therefore, the use of a high cost commercial inverted optical microscope is not indispensable. In this study, we designed a prototype of an optical inverted microscope created by 3D-printing and based on a smartphone. The system was evaluated with 226 MODS TB positive and 207 MODS TB negative digital images. These images were obtained from 10 sputum samples MODS positive and 10 sputum samples MODS negative. The quality of all images was assessed by a qualified technician, in terms of adequacy to interpret and classify them as positive or negative for tuberculosis. The quality of the images was considered appropriate for MODS interpretation. All the 20 samples were correctly classified (as TB positive/negative) by reading with the prototype 3D-printed inverted microscope.
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DOI：
10.1016/j.trstmh.2008.10.015
发表时间：
2009-06-01
影响因子：
2.2
作者：
Zimic, Mirko;Coronel, Jorge;Moore, David A. J.
通讯作者：
Moore, David A. J.
影响因子：
3.7
作者：
Morgan AJ;Hidalgo San Jose L;Jamieson WD;Wymant JM;Song B;Stephens P;Barrow DA;Castell OK
通讯作者：
Castell OK
影响因子：
9.4
作者：
Moore, DAJ;Mendoza, D;Serpa, J
通讯作者：
Serpa, J
影响因子：
3.7
作者：
Alva A;Aquino F;Gilman RH;Olivares C;Requena D;Gutiérrez AH;Caviedes L;Coronel J;Larson S;Sheen P;Moore DA;Zimic M
通讯作者：
Zimic M
开发低成本倒置显微镜来检测 MODS 培养物中结核分枝杆菌的早期生长。
影响因子：
3.7
作者：
Zimic M;Velazco A;Comina G;Coronel J;Fuentes P;Luna CG;Sheen P;Gilman RH;Moore DA
通讯作者：
Moore DA