Heterogeneous rates of molecular evolution among cryptic species of the ciliate morphospecies Chilodonella uncinata.
Heterogeneous rates of molecular evolution among cryptic species of the ciliate morphospecies Chilodonella uncinata.
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DOI：
10.1007/s00239-011-9468-x
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发表时间：
2011-12
影响因子：
3.9
通讯作者：
Muse, Spencer V.
中科院分区：
文献类型：
--
作者：
Katz, Laura A.;DeBerardinis, Jennifer;Hall, Meaghan S.;Kovner, Alexandra M.;Dunthorn, Micah;Muse, Spencer V.
虽然分子分析为纤毛虫的系统发育提供了洞察力，但少数评估种内变异的研究在很大程度上仅依赖于单个基因座(例如，核小亚基rDNA(nSSU-rDNA)或线粒体细胞色素氧化酶I)。在这项研究中，我们研究了纤毛虫形态种斑潜蝇的五个分离株的几个核蛋白编码基因以及nSSU-rDNA和线粒体小亚基rDNA(mtSSU-rDNA)的多样性。虽然这些分离株具有几乎相同的nSSU-rDNA序列，但它们在mtSSU-rDNA上的差异高达8.0%。对所有基因座的比较分析，包括β-微管蛋白平行序列，表明菌株之间缺乏重组，表明形态种钩藤由多个隐种组成。此外，不同基因座之间的替换率有相当大的差异，因为一些蛋白质编码区在分离株之间几乎相同，而另一些在氨基酸水平上的差异高达13.2%。结合对分离株之间的大核变异的见解，这项研究的重点，以及其中两个分离株的微核公布的数据表明，钩端螺旋藻的谱系能够在快速进化的种系基因组中同时保持高度差异和高度保守的基因。
While molecular analyses have provided insight into the phylogeny of ciliates, the few studies assessing intraspecific variation have largely relied on just a single locus (e.g. nuclear small subunit rDNA (nSSU-rDNA) or mitochondrial cytochrome oxidase I). In this study, we characterize the diversity of several nuclear protein-coding genes plus both nSSU-rDNA and mitochondrial small subunit rDNA (mtSSU-rDNA) of five isolates of the ciliate morphospecies Chilodonella uncinata. Although these isolates have nearly identical nSSU-rDNA sequences, they differ by up to 8.0% in mtSSU-rDNA. Comparative analyses of all loci, including β-tubulin paralogs, indicate a lack of recombination between strains, demonstrating that the morphospecies C. uncinata consists of multiple cryptic species. Further, there is considerable variation in substitution rates among loci as some protein-coding domains are nearly identical between isolates while others differ by up to 13.2% at the amino acid level. Combining insights on macronuclear variation among isolates, the focus of this study, with published data from the micronucleus of two of these isolates indicates that C. uncinata lineages are able to maintain both highly divergent and highly conserved genes within a rapidly evolving germline genome.
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影响因子：
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作者：
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