UPLC-HRMS-based untargeted metabolic profiling reveals changes in chickpea (Cicer arietinum) metabolome following long-term drought stress.
UPLC-HRMS-based untargeted metabolic profiling reveals changes in chickpea (Cicer arietinum) metabolome following long-term drought stress.
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DOI：
10.1111/pce.13195
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发表时间：
2019-01
期刊：
影响因子：
--
通讯作者：
Babar MA
中科院分区：
文献类型：
--
作者：
Khan N;Bano A;Rahman MA;Rathinasabapathi B;Babar MA
鹰嘴豆耐旱性的遗传改良需要对不同生理机制所涉及的生化过程有深入的了解。本研究的目的是通过基于高效液相色谱/高分辨率质谱的非靶向代谢组学分析，证明在不同水分条件下生长的鹰嘴豆品种（耐受性和敏感性）代谢水平改变的遗传变异。将鹰嘴豆植物在3叶期暴露于干旱胁迫25天，并在施加干旱胁迫后14天和25天收获叶片。胁迫导致叶绿素含量、Fv/Fm、相对含水量、地上部和根系干重显著降低。通过代谢物显著性分析和偏最小二乘判别分析两种方法，确定了20种已知代谢物为最重要的代谢物。干旱胁迫导致的最显著的积累增加表现为尿囊素、l-脯氨酸、l-精氨酸、l-组氨酸、l-异亮氨酸和色氨酸。在干旱条件下显示出降低的积累水平的代谢物是胆碱、苯丙氨酸、γ-氨基丁酸、丙氨酸、苯丙氨酸、酪氨酸、葡糖胺、鸟嘌呤和天冬氨酸。干旱条件下产生遗传变异的途径包括氨酰-tRNA和植物次生代谢产物的生物合成以及氨基酸的代谢或合成途径。干旱条件下的代谢变化突出了影响鹰嘴豆代谢和生理调节的代谢物库，减少了干旱的影响。干旱胁迫是鹰嘴豆种植区的主要问题之一。尽管干旱胁迫改变了植物的生化机制，但干旱胁迫下鹰嘴豆遗传改良的复杂代谢调控机制却知之甚少。本研究旨在鉴定干旱和对照条件下两种不同耐旱性鹰嘴豆品种不同代谢产物的变化。这项研究还表明了鹰嘴豆耐旱机制中可能涉及的代谢途径。
Genetic improvement for drought tolerance in chickpea requires a solid understanding of biochemical processes involved with different physiological mechanisms. The objective of this study is to demonstrate genetic variations in altered metabolic levels in chickpea varieties (tolerant and sensitive) grown under contrasting water regimes through ultrahigh‐performance liquid chromatography/high‐resolution mass spectrometry‐based untargeted metabolomic profiling. Chickpea plants were exposed to drought stress at the 3‐leaf stage for 25 days, and the leaves were harvested at 14 and 25 days after the imposition of drought stress. Stress produced significant reduction in chlorophyll content, F v/F m, relative water content, and shoot and root dry weight. Twenty known metabolites were identified as most important by 2 different methods including significant analysis of metabolites and partial least squares discriminant analysis. The most pronounced increase in accumulation due to drought stress was demonstrated for allantoin, l‐proline, l‐arginine, l‐histidine, l‐isoleucine, and tryptophan. Metabolites that showed a decreased level of accumulation under drought conditions were choline, phenylalanine, gamma‐aminobutyric acid, alanine, phenylalanine, tyrosine, glucosamine, guanine, and aspartic acid. Aminoacyl‐tRNA and plant secondary metabolite biosynthesis and amino acid metabolism or synthesis pathways were involved in producing genetic variation under drought conditions. Metabolic changes in light of drought conditions highlighted pools of metabolites that affect the metabolic and physiological adjustment in chickpea that reduced drought impacts. Drought stress is one of the major problems in chickpea‐growing areas. Though drought stress changes biochemical mechanisms in plants, however, little is known about the complex metabolic regulation for genetic improvement in chickpea under drought stress environments. This study was conducted to identify changes at different metabolites in two chickpea varieties contrasting for drought tolerance under drought and control conditions. This study also demonstrates the metabolic pathways potentially involved in drought tolerance mechanisms in chickpea.
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影响因子：
3.7
作者：
Ahmed MM;Dhanasekaran AR;Block A;Tong S;Costa AC;Stasko M;Gardiner KJ
通讯作者：
Gardiner KJ
DOI：
10.1051/agro:2008021
发表时间：
2009-01-01
期刊：
SUSTAINABLE AGRICULTURE
影响因子：
--
作者：
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作者：
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Wang LC
影响因子：
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作者：
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