Molecular cloning of protamine-2 and expression with aging in Japanese monkey (Macaca fuscata)
Molecular cloning of protamine-2 and expression with aging in Japanese monkey (Macaca fuscata)
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日本猴 (Macaca fuscata) 鱼精蛋白 2 的分子克隆及其衰老表达
DOI：
10.1007/s10329-003-0069-1
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发表时间：
2004
期刊：
影响因子：
1.7
通讯作者：
O. Takenaka
中科院分区：
文献类型：
--
作者：
Sungsook Yu;O. Takenaka
为了分离精子发生与出生后发育和衰老相关的基因，我们试图通过差异显示聚合酶链式反应(Differential Display PCR)获得日本猕猴(Macaca Fuscata)睾丸表达差异的基因，并对日本猕猴精蛋白-2(Protamine-2，PRM2)进行克隆、测序和鉴定。预测的开放阅读框编码一个103个氨基酸残基的蛋白质，其中大部分在其他哺乳动物中是共同的。Northern分析表明，PRM2基因在成年期和老年期都有表达，但在幼年期不表达。原位杂交显示，PRM2基因主要在晚期精子细胞中表达，其表达能力从成年期到老年期逐渐降低。提示PRM2在精子发生中的作用受年龄的影响。
In order to isolate genes relating spermatogenesis with postnatal development and aging, we have attempted to obtain genes showing differences in expression in testis of Japanese monkeys (Macaca fuscata) by means of differential display PCR, and we have cloned, sequenced and characterized protamine-2 (PRM2) of Japanese monkey. The predicted open reading frame encoded a protein of 103 amino acid residues, most of which are common to those of other mammals. Northern analysis revealed that the PRM2 gene is expressed at adult and aged stages, but not at the juvenile stage. In situ hybridization revealed that the PRM2 gene is expressed mainly in late spermatids and its expressional potential is decreased from adult to aged stages. It suggests that PRM2 in spermiogenesis is mediated by the age of the animal.
年龄对男性精子产生和睾丸重量的影响。
DOI：
10.1530/jrf.0.0700211
发表时间：
1984
期刊：
Journal of reproduction and fertility
影响因子：
--
作者：
Johnson,L;Petty,CS;Neaves,WB
通讯作者：
Neaves,WB
影响因子：
5.8
作者：
NEAVES, WB;JOHNSON, L;PETTY, CS
通讯作者：
PETTY, CS