Genome Analysis of Two Pseudonocardia Phylotypes Associated with Acromyrmex Leafcutter Ants Reveals Their Biosynthetic Potential.
Genome Analysis of Two Pseudonocardia Phylotypes Associated with Acromyrmex Leafcutter Ants Reveals Their Biosynthetic Potential.
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DOI：
10.3389/fmicb.2016.02073
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发表时间：
2016
影响因子：
5.2
通讯作者：
Hutchings MI
中科院分区：
文献类型：
--
作者：
Holmes NA;Innocent TM;Heine D;Bassam MA;Worsley SF;Trottmann F;Patrick EH;Yu DW;Murrell JC;Schiøtt M;Wilkinson B;Boomsma JJ;Hutchings MI
南美洲和中美洲的蚂蚁是古老的农民，在5000万年前进化出与真菌食物作物的共生关系。最具进化意义的蚁是阿塔蚁和Acromyrmex切叶蚁，它们收割新鲜的叶子来喂养它们的真菌。Acromyrmex和许多其他attines垂直传播假诺卡氏菌的互惠菌株，并使用这些细菌产生的抗真菌化合物来保护它们的真菌伴侣免受Escovopsis属的共同进化的真菌病原体的侵害。与Apterostigma dentigerum和Trachymyrmex corntzi相关的Pseudonocardia mutualists产生了称为gerumycins的新型环状缩肽化合物，而从Acromyrmex spinosus中分离出的mutualists菌株产生了称为制霉菌素P1的不寻常的多烯抗真菌剂。这些抗菌药物的新奇表明，在全基因组范围内探索假诺卡氏菌的次级代谢产物是有价值的。在这里，我们报告的基因组分析的Pseudonocardia peptides型Ps1和Ps2，一贯与Acromyrmex蚂蚁收集在甘博阿，巴拿马。这些先前仅根据16S rRNA基因测序进行区分，但5个Ps1和5个Ps2菌株的基因组测序显示，这些Ps1和Ps2类型是不同的物种，每个编码11至15个次级代谢物生物合成基因簇（BGC）。Ps1和Ps2菌株有特征BGC，有些在两者中都是保守的。Ps1菌株都含有编码制霉菌素P1样抗真菌剂的BGC，而Ps2菌株编码新的制霉菌素样分子。菌株显示这些BGC排列的变化，类似于在gerumycin基因簇中看到的那些。基因组分析和侵入试验支持我们的假设，即垂直传播的Ps1和Ps2菌株具有抗菌活性，可以帮助塑造表皮微生物组。因此，我们的工作定义了与Acromyrmex蚂蚁相关的假诺卡氏菌属物种，并支持假诺卡氏菌属物种可能提供新的抗菌剂的有价值的来源的假设。
The attine ants of South and Central America are ancient farmers, having evolved a symbiosis with a fungal food crop >50 million years ago. The most evolutionarily derived attines are the Atta and Acromyrmex leafcutter ants, which harvest fresh leaves to feed their fungus. Acromyrmex and many other attines vertically transmit a mutualistic strain of Pseudonocardia and use antifungal compounds made by these bacteria to protect their fungal partner against co-evolved fungal pathogens of the genus Escovopsis. Pseudonocardia mutualists associated with the attines Apterostigma dentigerum and Trachymyrmex cornetzi make novel cyclic depsipeptide compounds called gerumycins, while a mutualist strain isolated from derived Acromyrmex octospinosus makes an unusual polyene antifungal called nystatin P1. The novelty of these antimicrobials suggests there is merit in exploring secondary metabolites of Pseudonocardia on a genome-wide scale. Here, we report a genomic analysis of the Pseudonocardia phylotypes Ps1 and Ps2 that are consistently associated with Acromyrmex ants collected in Gamboa, Panama. These were previously distinguished solely on the basis of 16S rRNA gene sequencing but genome sequencing of five Ps1 and five Ps2 strains revealed that the phylotypes are distinct species and each encodes between 11 and 15 secondary metabolite biosynthetic gene clusters (BGCs). There are signature BGCs for Ps1 and Ps2 strains and some that are conserved in both. Ps1 strains all contain BGCs encoding nystatin P1-like antifungals, while the Ps2 strains encode novel nystatin-like molecules. Strains show variations in the arrangement of these BGCs that resemble those seen in gerumycin gene clusters. Genome analyses and invasion assays support our hypothesis that vertically transmitted Ps1 and Ps2 strains have antibacterial activity that could help shape the cuticular microbiome. Thus, our work defines the Pseudonocardia species associated with Acromyrmex ants and supports the hypothesis that Pseudonocardia species could provide a valuable source of new antimicrobials.
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影响因子：
4.9
作者：
Andersen SB;Hansen LH;Sapountzis P;Sørensen SJ;Boomsma JJ
通讯作者：
Boomsma JJ
影响因子：
2.1
作者：
Karki, Suman;Kwon, So-Yeon;Kwon, Hyung-Jin
通讯作者：
Kwon, Hyung-Jin
影响因子：
2.6
作者：
Gallucci, M. N.;Oliva, M.;Demo, M.
通讯作者：
Demo, M.
影响因子：
15
作者：
GROSS, E;MORELL, JL
通讯作者：
MORELL, JL
DOI：
10.1007/s10295-009-0629-5
发表时间：
2009-11-01
影响因子：
3.4
作者：
Kim, Byung-Gyun;Lee, Mi-Jin;Kim, Eung-Soo
通讯作者：
Kim, Eung-Soo