Specificity and stability of the Acromyrmex-Pseudonocardia symbiosis.
Specificity and stability of the Acromyrmex-Pseudonocardia symbiosis.
复制标题
DOI：
10.1111/mec.12380
复制
发表时间：
2013-08
影响因子：
4.9
通讯作者：
Boomsma JJ
中科院分区：
文献类型：
--
作者：
Andersen SB;Hansen LH;Sapountzis P;Sørensen SJ;Boomsma JJ
互惠相互作用的稳定性可能受到竞争相同功能生态位的共生体遗传多样性的影响。真菌生长（attine）蚂蚁具有多种复杂的共生体，因此为解决共生体特异性和多样性问题提供了充足的机会。这些伙伴包括假诺卡氏菌属的放线菌，它们维持在蚂蚁角质层上以产生抗生素，主要针对共生花园的真菌寄生虫。共生被认为是进化稳定性的标志，但这一观点受到了培养和测序数据的挑战，这些数据表明存在不可预测的高度多样性。我们使用 16S rRNA 的 454 焦磷酸测序来估计来自巴拿马甘博阿的切叶蚁 Acromyrmex echinatior 和其他真菌生长蚂蚁的角质层细菌群落的多样性。收集了同一菌落的现场和实验室样本，后者是在菌落在实验室条件下保存长达 10 年之后收集的。我们证明细菌群落具有高度的菌落特异性，并且随着时间的推移保持稳定。大多数菌落 (25/26) 具有单一优势假诺卡氏菌菌株，而 17 年来在甘博阿种群中只发现了两种菌株，这证实了一项早期研究。新孵化的蚂蚁上的微生物群落几乎完全由假诺卡氏菌单一菌株组成，而在较老的觅食蚂蚁上发现了其他放线菌，其丰度各不相同，但通常要低得多。这些发现与最近的理论一致，该理论预测，当单一垂直传播且需要资源的菌株占主导地位时，产生抗生素的细菌混合物可以保持互利共生。
The stability of mutualistic interactions is likely to be affected by the genetic diversity of symbionts that compete for the same functional niche. Fungus-growing (attine) ants have multiple complex symbioses and thus provide ample opportunities to address questions of symbiont specificity and diversity. Among the partners are Actinobacteria of the genus Pseudonocardia that are maintained on the ant cuticle to produce antibiotics, primarily against a fungal parasite of the mutualistic gardens. The symbiosis has been assumed to be a hallmark of evolutionary stability, but this notion has been challenged by culturing and sequencing data indicating an unpredictably high diversity. We used 454 pyrosequencing of 16S rRNA to estimate the diversity of the cuticular bacterial community of the leaf-cutting ant Acromyrmex echinatior and other fungus-growing ants from Gamboa, Panama. Both field and laboratory samples of the same colonies were collected, the latter after colonies had been kept under laboratory conditions for up to 10 years. We show that bacterial communities are highly colony-specific and stable over time. The majority of colonies (25/26) had a single dominant Pseudonocardia strain, and only two strains were found in the Gamboa population across 17 years, confirming an earlier study. The microbial community on newly hatched ants consisted almost exclusively of a single strain of Pseudonocardia while other Actinobacteria were identified on older, foraging ants in varying but usually much lower abundances. These findings are consistent with recent theory predicting that mixtures of antibiotic-producing bacteria can remain mutualistic when dominated by a single vertically transmitted and resource-demanding strain.
登录
查看更多内容
影响因子：
3.3
作者：
Bot, ANM;Rehner, SA;Boomsma, JJ
通讯作者：
Boomsma, JJ
影响因子：
9.8
作者：
Gerardo, Nicole M.;Jacobs, Sarah R.;Mueller, Ulrich G.
通讯作者：
Mueller, Ulrich G.
影响因子：
2.9
作者：
Archetti, Marco;Ubeda, Francisco;Yu, Douglas W.
通讯作者：
Yu, Douglas W.
DOI：
10.1098/rspb.2001.1605
发表时间：
2001-05-22
影响因子：
4.7
作者：
Currie, CR;Stuart, AE
通讯作者：
Stuart, AE
影响因子：
64.8
作者：
Currie, CR;Scott, JA;Malloch, D
通讯作者：
Malloch, D