Analysis of T cell hybridomas. II. Comparisons among three distinct types of monoclonal suppressor factors
Analysis of T cell hybridomas. II. Comparisons among three distinct types of monoclonal suppressor factors
复制标题
T 细胞杂交瘤分析。
DOI：
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发表时间：
1981
影响因子：
15.3
通讯作者：
M. Dorf
中科院分区：
文献类型：
--
作者：
Kenji Okuda;Mutsuhiko Minami;Shuichi Furusawa;M. Dorf
将TS3细胞与BW5147胸腺瘤融合，获得5株杂交瘤T细胞株。这些T细胞杂交瘤细胞的培养上清液中含有一种因子TsF3，它能特异性地抑制4-羟基-3-硝基苯乙酰半抗原(NP)皮肤过敏反应。将这一系列新杂交瘤因子的性质与先前鉴定的两种NP特异性抑制因子(TsF1和TsF2)的性质进行了比较。只有在免疫反应的效应阶段给予该因子时，才能观察到TsF3的活性。TsF3含有I-J和C57BL抗NP抗体独特型决定簇，对NP半抗原具有结合特异性。此外，TsF3不抑制H-2(I-J)血型不合的小鼠。除了H-2限制外，克隆的TsF3因子也表现出高水平的遗传限制。最后，TsF3因子可以通过它们抑制环磷酰胺治疗的受体的能力来区分。
Five hybridoma T cell lines were prepared by fusion of Ts3 cells with the BW 5147 thymoma. The culture supernatants from these T cell hybrids contained a factor, TsF3, which specifically suppressed 4-hydroxy-3- nitrophenyl acetyl hapten (NP(-hapten cutaneous sensitivity responses. The properties of this new series of hybridoma factors was compared with those of two previously characterized types of NP-specific suppressor factors (TsF1 and TsF2). TsF3 activity was only observed if the factor was administered during the effector phases of the immune response. TsF3 bears I-J and C57BL anti-NP antibody idiotypic determinants and has binding specificity for the NP hapten. Furthermore, TsF3 does not suppress H-2 (I-J)-incompatible mice. In addition to this H-2 restriction, the monoclonal TsF3 factors also demonstrated an Igh genetic restriction. Finally, the TsF3 factors could be distinguished by their ability to suppress cyclophosphamide- treated recipients.
来自 T 细胞杂交体的抑制因子可抑制对偶氮苯胂酸盐的迟发型超敏反应。
DOI：
10.1073/pnas.78.10.6441
发表时间：
1981
影响因子：
11.1
作者：
Whitaker,RB;Nepom,JT;Sy,MS;Takaoki,M;Gramm,CF;Fox,I;Germain,RN;Nelles,MJ;Greene,MI;Benacerraf,B
通讯作者：
Benacerraf,B