Adipose derived stem cells ameliorate hyperlipidemia associated detrusor overactivity in a rat model.
Adipose derived stem cells ameliorate hyperlipidemia associated detrusor overactivity in a rat model.
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DOI：
10.1016/j.juro.2009.11.012
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发表时间：
2010-03
期刊：
影响因子：
--
通讯作者：
Lin CS
中科院分区：
文献类型：
--
作者：
Huang YC;Shindel AW;Ning H;Lin G;Harraz AM;Wang G;Garcia M;Lue TF;Lin CS
脂肪组织源性干细胞（ADSC）可以在体外分化为肌肉和神经元样细胞。在这项研究中，我们探讨了ADSC在治疗肥胖高脂血症大鼠（OHR）膀胱过动症（OAB）中的作用。健康大鼠高脂饮食诱导高脂血症。然后用膀胱注射生理盐水或ADSC或尾静脉注射ADSC治疗OHR。通过24小时排尿行为研究和有意识膀胱测量来评估膀胱功能。采用免疫染色、三色染色及图像分析评估膀胱组织学。OHR大鼠血清总胆固醇和低密度脂蛋白水平显著高于正常大鼠（p < 0.01）。与正常大鼠、经尾静脉注射ADSC的大鼠和膀胱注射ADSC的大鼠相比，经盐水处理的大鼠排尿间隔时间更短（143±28.7秒vs 407±77.9秒vs 281±43.9秒vs 368±66.7秒，p = 0.0084）。OHR组膀胱壁平滑肌含量显著低于正常组（p = 0.0061），而OHR组间差异无统计学意义。对照组的神经含量和血管密度低于adsc治疗的OHR。高脂血症与大鼠尿频增加、膀胱血管和神经密度降低有关。ADSC治疗改善了这些不良反应，有望成为OAB的潜在新疗法。
It has been previously demonstrated that adipose tissue-derived stem cell (ADSC) can differentiate into muscle and neuron-like cells in vitro. In this study, we investigate the utility of ADSC in the treatment of overactive bladder (OAB) in obese hyperlipidemic rats (OHR). Hyperlipidemia was induced in healthy rats by administration of a high fat diet. The resulting OHR were then treated with bladder injection of saline or ADSC or tail vein injection of ADSC. Bladder function was assessed by 24-h voiding behavior study and conscious cystometry. Bladder histology was assessed using immunostaining and trichrome staining followed by image analysis. Serum total cholesterol and low-density lipoprotein levels were significantly higher in OHR than in normal rats (p < 0.01). Micturition intervals were shorter in the saline-treated OHR relative to normal rats, OHR that received ADSC via tail vein, and OHR that received ADSC by bladder injection (143 ± 28.7 vs 407 ± 77.9 vs 281 ± 43.9 vs 368 ± 66.7 seconds respectively, p = 0.0084). Smooth muscle content of the bladder wall was significantly lower in OHR than in normal animals (p = 0.0061) while there was no significant difference between OHR groups. Nerve content and blood vessel density were lower in control than in ADSC-treated OHR. Hyperlipidemia is associated with increased urinary frequency and diminished bladder blood vessel and nerve density in rats. Treatment with ADSC ameliorates these adverse effects and holds promise as a potential new therapy for OAB.
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影响因子：
2
作者：
GORMLEY, EA;GRIFFITHS, DJ;MCPHEE, MS
通讯作者：
MCPHEE, MS
影响因子：
2.4
作者：
Housman, TS;Lawrence, N;Fleischer, AB
通讯作者：
Fleischer, AB
影响因子：
37.8
作者：
Miranville, A;Heeschen, C;Bouloumié, A
通讯作者：
Bouloumié, A
DOI：
10.1073/pnas.0510511103
发表时间：
2006-01-31
影响因子：
11.1
作者：
Spees, JL;Olson, SD;Prockop, DJ
通讯作者：
Prockop, DJ
影响因子：
37.8
作者：
Rehman, J;Traktuev, D;March, KL
通讯作者：
March, KL