Exosome encased spherical nucleic acid gold nanoparticle conjugates as potent microRNA regulation agents.
Exosome encased spherical nucleic acid gold nanoparticle conjugates as potent microRNA regulation agents.
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DOI：
10.1002/smll.201302143
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发表时间：
2014-01-15
期刊：
影响因子：
13.3
通讯作者：
Mirkin, Chad A.
中科院分区：
文献类型：
--
作者：
Alhasan, Ali H.;Patel, Pinal C.;Choi, Chung Hang J.;Mirkin, Chad A.
外泌体是一类天然存在的纳米材料，在微RNA和mRNA等内源性大分子的长距离保护和运输中发挥着至关重要的作用。目前正在加紧努力利用外泌体来递送合成治疗剂。在本文中，我们使用透射电子显微镜来显示，当球形核酸（SNA）构建体被内吞到PC-3前列腺癌细胞中时，它们中的一小部分（< 1%）可以自然地分选到外泌体中。外泌体包裹的SNA分泌到细胞外环境中，它们可以从细胞外环境中分离并选择性地重新引入到它们所来源的细胞类型中。在抗miR-21实验的背景下，外泌体包裹的SNA敲低miR-21靶标约50%。通过游离SNA类似地敲低miR-21需要高约3000倍的浓度。
Exosomes are a class of naturally occurring nanomaterials that play crucial roles in the protection and transport of endogenous macromolecules, such as microRNA and mRNA, over long distances. Intense effort is underway to exploit the use of exosomes to deliver synthetic therapeutics. Herein, we use transmission electron microscopy to show that when spherical nucleic acid (SNA) constructs are endocytosed into PC-3 prostate cancer cells, a small fraction of them (< 1%) can be naturally sorted into exosomes. The exosome-encased SNAs are secreted into the extracellular environment from which they can be isolated and selectively re-introduced into the cell type from which they were derived. In the context of anti-miR21 experiments, the exosome-encased SNAs knockdown miR-21 target by approximately 50%. Similar knockdown of miR-21 by free SNAs requires a ~3000-fold higher concentration.
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影响因子：
46.9
作者：
Alvarez-Erviti, Lydia;Seow, Yiqi;Wood, Matthew J. A.
通讯作者：
Wood, Matthew J. A.
使用球形核酸 - 金纳米粒子结合物的前列腺癌标记物的扫描microRNA阵列分析。
影响因子：
7.4
作者：
Alhasan, Ali H.;Kim, Dae Y.;Daniel, Weston L.;Watson, Erin;Meeks, Joshua J.;Thaxton, C. Shad;Mirkin, Chad A.
通讯作者：
Mirkin, Chad A.
影响因子：
17.1
作者：
Prigodich, Andrew E.;Seferos, Dwight S.;Massich, Matthew D.;Giljohann, David A.;Lane, Brandon C.;Mirkin, Chad A.
通讯作者：
Mirkin, Chad A.
影响因子：
4.7
作者：
Agbasi-Porter, Chiamaka;Ryman-Rasmussen, Jessica;Feldheim, Daniel
通讯作者：
Feldheim, Daniel
影响因子：
56.9
作者：
CHATTERJEE, S;JOHNSON, PR;WONG, KK
通讯作者：
WONG, KK