Ultrashort echo time (UTE) imaging with bi-component analysis: bound and free water evaluation of bovine cortical bone subject to sequential drying.
Ultrashort echo time (UTE) imaging with bi-component analysis: bound and free water evaluation of bovine cortical bone subject to sequential drying.
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DOI：
10.1016/j.bone.2011.11.029
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发表时间：
2012-03
期刊：
影响因子：
4.1
通讯作者：
Du, Jiang
中科院分区：
文献类型：
--
作者：
Biswas, Reni;Bae, Won;Diaz, Eric;Masuda, Koichi;Chung, Christine B.;Bydder, Graeme M.;Du, Jiang
最近的质子磁共振（MR）波谱研究表明，皮质骨作为不同的成分存在，具有不同的横向弛豫时间（T2）。然而，在临床扫描仪上使用所有常规MR序列时，皮质骨显示为零或接近零信号，并且使用该方法无法评估不同的水成分。为了检测这种情况下的信号，使用标称TE为8 μs的二维（2D）非切片选择性超短回波时间（UTE）脉冲序列以及双组分分析，以在3 T下定量牛皮质骨中的结合水和游离水。使用3D UTE序列和参考水体模对总水浓度进行定量。对14个牛骨样品进行2D和3D UTE成像，这些牛骨样品进行连续的空气干燥以评估游离水损失，然后进行烘箱干燥以评估结合水损失。使用精密天平同时测量连续骨重量损失。骨孔隙率用微型计算机断层扫描（μCT）成像测量。UTE测量的游离水损失高于μCT测量的皮质孔体积，但低于空气干燥期间测量的重量骨水分损失。在烘箱干燥过程中，UTE评估的结合水损失约为重量骨水损失的82%。平均牛皮质骨显示约13%的自由水和87%的结合水。在连续空气干燥过程中，UTE MR测量的游离水损失和重量骨重量损失之间存在高度相关性（R = 0.91; P < 0.0001），在烘箱干燥过程中，UTE结合水损失和重量骨重量损失之间存在显著相关性（R = 0.69; P < 0.01）。这些结果表明，UTE双组分分析可以可靠地量化皮质骨中的结合水和自由水。该技术具有潜在的应用在体内评价骨孔隙度和有机基质。
Recent proton magnetic resonance (MR) spectroscopy studies have shown that cortical bone exists as different components which have distinct transverse relaxation times (T2s). However, cortical bone shows zero or near zero signal with all conventional MR sequences on clinical scanners and the different water components cannot be assessed with this approach. In order to detect signal in this situation a two-dimensional (2D) non-slice selective ultrashort echo time (UTE) pulse sequence with a nominal TE of 8 μs was used together with bi-component analysis to quantify bound and free water in bovine cortical bone at 3T. Total water concentration was quantified using a 3D UTE sequence together with a reference water phantom. 2D and 3D UTE imaging were performed on 14 bovine bone samples which were subjected to sequential air-drying to evaluate free water loss, followed by oven-drying to evaluate bound water loss. Sequential bone weight loss was measured concurrently using a precision balance. Bone porosity was measured with micro computed tomography (μCT) imaging. UTE measured free water loss was higher than the volume of cortical pores measured with μCT, but lower than the gravimetric bone water loss measured during air-drying. UTE assessed bound water loss was about 82% of gravimetric bone water loss during oven-drying. On average bovine cortical bone showed about 13% free water and 87% bound water. There was a high correlation (R = 0.91; P < 0.0001) between UTE MR measured free water loss and gravimetric bone weight loss during sequential air-drying, and a significant correlation (R = 0.69; P < 0.01) between UTE bound water loss and gravimetric bone weight loss during oven-drying. These results show that UTE bi-component analysis can reliably quantify bound and free water in cortical bone. The technique has potential applications for the in vivo evaluation of bone porosity and organic matrix.
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影响因子：
4.1
作者：
Cao, Haihui;Nazarian, Ara;Ackerman, Jerome L.;Snyder, Brian D.;Rosenberg, Andrew E.;Nazarian, Rosalynn M.;Hrovat, Mirko I.;Dai, Guangping;Mintzopoulos, Dionyssios;Wu, Yaotang
通讯作者：
Wu, Yaotang
影响因子：
4.2
作者：
LEES, S
通讯作者：
LEES, S
影响因子：
2.4
作者：
Ni, Qingwen;Nyman, Jeffry S.;Nicolella, Daniel P.
通讯作者：
Nicolella, Daniel P.
影响因子：
3.3
作者：
Horch, R. Adam;Nyman, Jeffry S.;Gochberg, Daniel F.;Dortch, Richard D.;Does, Mark D.
通讯作者：
Does, Mark D.
影响因子：
3.3
作者：
Carl, Michael;Bydder, Mark;Han, Eric
通讯作者：
Han, Eric