Quantitative (31)P NMR spectroscopy and (1)H MRI measurements of bone mineral and matrix density differentiate metabolic bone diseases in rat models.
Quantitative (31)P NMR spectroscopy and (1)H MRI measurements of bone mineral and matrix density differentiate metabolic bone diseases in rat models.
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DOI：
10.1016/j.bone.2010.02.020
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发表时间：
2010-06
期刊：
影响因子：
4.1
通讯作者：
Wu, Yaotang
中科院分区：
文献类型：
--
作者：
Cao, Haihui;Nazarian, Ara;Ackerman, Jerome L.;Snyder, Brian D.;Rosenberg, Andrew E.;Nazarian, Rosalynn M.;Hrovat, Mirko I.;Dai, Guangping;Mintzopoulos, Dionyssios;Wu, Yaotang
在这项研究中，通过 31P NMR 波谱测量了正常 (CON)、卵巢切除 (OVX) 和部分肾切除 (NFR) 大鼠的骨矿物质密度 (BMD)；通过1H水和脂肪抑制投影成像（WASPI）测量骨基质密度；骨矿化程度(EBM)通过BMD/骨基质密度的比值得到。根据化学分析（重量分析）评估这些 MR 方法区分 CON、OVX 和 NFR 组骨成分的能力。对于皮质骨标本，CON组和OVX组的BMD没有显着差异； NFR组的BMD比CON低22.1%（通过31P NMR）和17.5%（通过重量分析）。对于小梁骨标本，OVX 组的 BMD 比 CON 低 40.5%（通过 31P NMR）和 24.6%（通过重量分析）； NFR组的BMD比CON低26.8%（通过31P NMR）和21.5%（通过重量分析）。 WASPI或重量分析未观察到CON和OVX之间皮质骨基质密度的显着变化； NFR 皮质骨基质密度比 CON 低 10.3%（通过 WASPI）和 13.9%（通过重量分析）。 OVX 骨小梁基质密度比 CON 低 38.0%（通过 WASPI）和 30.8%（通过重量分析），而两种方法均未观察到 NFR 小梁骨基质密度的显着变化。 OVX 皮质和小梁标本的 EBM 略高于 CON，但与 CON 没有显着差异。重要的是，通过 31P NMR/WASPI 检测，NFR 皮质和小梁样本的 EBM 分别比 CON 低 12.4% 和 26.3%；通过重量分析法，分别降低了 4.0% 和 11.9%。组织病理学显示 OVX 组有骨质疏松症，NFR 组有严重继发性甲状旁腺功能亢进（肾性骨营养不良）。这些结果表明，31P NMR/WASPI 组合方法能够辨别骨质疏松动物和骨矿化受损动物之间 EBM 的差异。
In this study, bone mineral density (BMD) of normal (CON), ovariectomized (OVX) and partially nephrectomized (NFR) rats was measured by 31P NMR spectroscopy; bone matrix density was measured by 1H water- and fat-suppressed projection imaging (WASPI); and the extent of bone mineralization (EBM) was obtained by the ratio of BMD/bone matrix density. The capability of these MR methods to distinguish the bone composition of the CON, OVX and NFR groups was evaluated against chemical analysis (gravimetry). For cortical bone specimens, BMD of the CON and OVX groups was not significantly different; BMD of the NFR group was 22.1% (by 31P NMR) and 17.5% (by gravimetry) lower than CON. For trabecular bone specimens, BMD of the OVX group was 40.5% (by 31P NMR) and 24.6% (by gravimetry) lower than CON; BMD of the NFR group was 26.8% (by 31P NMR) and 21.5% (by gravimetry) lower than CON. No significant change of cortical bone matrix density between CON and OVX was observed by WASPI or gravimetry; NFR cortical bone matrix density was 10.3% (by WASPI) and 13.9% (by gravimetry) lower than CON. OVX trabecular bone matrix density was 38.0% (by WASPI) and 30.8% (by gravimetry) lower than CON, while no significant change in NFR trabecular bone matrix density was observed by either method. The EBMs of OVX cortical and trabecular specimens were slightly higher than CON but not significantly different from CON. Importantly, EBMs of NFR cortical and trabecular specimens were 12.4% and 26.3% lower than CON by 31P NMR/WASPI, respectively; and 4.0% and 11.9% lower by gravimetry. Histopathology showed evidence of osteoporosis in the OVX group and severe secondary hyperparathyroidism (renal osteodystrophy) in the NFR group. These results demonstrate that the combined 31P NMR/WASPI method is capable of discerning the difference in EBM between animals with osteoporosis and those with impaired bone mineralization.
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