Injectable bone tissue engineering using expanded mesenchymal stem cells.
Injectable bone tissue engineering using expanded mesenchymal stem cells.
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DOI：
10.1002/stem.1300
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发表时间：
2013-03
期刊：
影响因子：
5.2
通讯作者：
Wakabayashi, Toshihiko
中科院分区：
文献类型：
--
作者：
Yamada, Yoichi;Nakamura, Sayaka;Ito, Kenji;Umemura, Eri;Hara, Kenji;Nagasaka, Tetsuro;Abe, Akihiro;Baba, Shunsuke;Furuichi, Yasushi;Izumi, Yuichi;Klein, Ophir D.;Wakabayashi, Toshihiko
患有骨缺损的患者通常采用自体骨移植进行治疗，但这种疗法可能导致许多并发症。因此，需要新的方法来改善骨缺损的治疗，而干细胞治疗提供了一种令人兴奋的替代方法。虽然已经报道了来自使用干细胞的基础研究的广泛证据，但是很少开发使用干细胞用于骨组织工程的临床应用。我们研究了由间充质干细胞（MSCs）和富含血小板的血浆组成的可注射组织工程骨是否能够在牙槽骨缺损中再生功能性骨。我们在动物中进行了这些研究，随后在长期随访的患者中进行了试点试验;这些研究表明，使用微创MSC移植可以实现良好的骨形成。所有患者的骨体积均显著改善，无副作用。术后3个月新骨形成面积显著高于术前基线（P <0.001），达到与自体骨相当的水平。长期随访无明显骨吸收。可注射组织工程骨修复患者咀嚼功能。这种新的临床方法代表了骨组织工程的有效治疗利用。
Patients suffering from bone defects are often treated with autologous bone transplants, but this therapy can cause many complications. New approaches are therefore needed to improve treatment for bone defects, and stem cell therapy presents an exciting alternative approach. Although extensive evidence from basic studies using stem cells has been reported, very few clinical applications using stem cells for bone tissue engineering have been developed. We investigated whether injectable tissue-engineered bone composed of mesenchymal stem cells (MSCs) and platelet rich plasma was able to regenerate functional bone in alveolar deficiencies. We performed these studies in animals and subsequently carried out pilot trial cases in patients with long-term follow up; these showed good bone formation using minimally invasive MSC transplantation. All patients exhibited significantly improved bone volume with no side effects. Newly formed bone areas at 3 months was significantly higher than the pre-operation baseline (P <0.001) and reached levels equivalent to that of native bone. No significant bone resorption occurred during long term follow-up. Injectable tissue-engineered bone restored masticatory function in patients. This novel clinical approach represents an effective therapeutic utilization of bone tissue engineering.
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影响因子：
3.5
作者：
Brennan, David S.;Spencer, A. John;Roberts-Thomson, Kaye F.
通讯作者：
Roberts-Thomson, Kaye F.
影响因子：
3.3
作者：
Johansson, B;Grepe, A;Hirsch, JM
通讯作者：
Hirsch, JM
DOI：
10.1016/s1079-2104(98)90029-4
发表时间：
1998-06-01
影响因子：
--
作者：
Marx, RE;Carlson, ER;Georgeff, KR
通讯作者：
Georgeff, KR
影响因子：
1.9
作者：
Karamouzian, Saeid;Nematollahi-Mahani, Seyed Noureddin;Eskandary, Hossein
通讯作者：
Eskandary, Hossein
影响因子：
4.3
作者：
Norton, MR;Gamble, C
通讯作者：
Gamble, C