Genomic mapping of 5-hydroxymethylcytosine in the human brain.
Genomic mapping of 5-hydroxymethylcytosine in the human brain.
复制标题
DOI：
10.1093/nar/gkr120
复制
发表时间：
2011-07
影响因子：
14.9
通讯作者：
Pfeifer GP
中科院分区：
文献类型：
--
作者：
Jin SG;Wu X;Li AX;Pfeifer GP
胞嘧啶5位的甲基化是一种研究充分的表观遗传途径。除了5-甲基胞嘧啶（5 mC），在某些组织中也检测到大量的5-羟甲基胞嘧啶（5 hmC），也称为第六个DNA碱基，最明显的是大脑。然而，这种胞嘧啶修饰的基因组分布是未知的。在这里，我们已经使用了免疫沉淀技术（5 hmC-IP），以检查5 hmC的出现在DNA从人脑额叶组织。将5 hmC的分布与5 mC的分布进行比较。我们发现，5 hmC是更有选择性地针对基因比5 mC。5 hmC在启动子和基因内区域（基因体）特别富集，但在非基因区域基本不存在。转录起始位点的5 hmC峰与具有中等或高CpG含量的启动子的基因表达水平无关。然而，5 hmC基因体的存在与基因表达水平的正相关性比5 mC。睾丸特异性基因的启动子在脑DNA中显示出强烈的5 mC峰，但几乎完全没有5 hmC。我们的数据提供了人类大脑中5 hmC的基因组分布的概述，并将为这种新型DNA修饰的进一步功能表征奠定基础。
Methylation at the 5-position of cytosine is a well-studied epigenetic pathway. In addition to 5-methylcytosine (5mC), substantial amounts of 5-hydroxymethylcytosine (5hmC) also referred to as the sixth DNA base have been detected in certain tissues, most notably the brain. However, the genomic distribution of this cytosine modification is unknown. Here, we have used an immunoprecipitation technique (5hmC-IP) to examine the occurrence of 5hmC in DNA from human brain frontal lobe tissue. The distribution of 5hmC was compared to that of 5mC. We show that 5hmC is more selectively targeted to genes than is 5mC. 5hmC is particularly enriched at promoters and in intragenic regions (gene bodies) but is largely absent from non-gene regions. 5hmC peaks at transcription start sites did not correlate with gene expression levels for promoters with intermediate or high CpG content. However, the presence of 5hmC in gene bodies was more positively correlated with gene expression levels than was the presence of 5mC. Promoters of testis-specific genes showed strong 5mC peaks in brain DNA but were almost completely devoid of 5hmC. Our data provide an overview of the genomic distribution of 5hmC in human brain and will set the stage for further functional characterization of this novel DNA modification.
登录
查看更多内容
影响因子：
14.9
作者：
Jin SG;Kadam S;Pfeifer GP
通讯作者：
Pfeifer GP
DOI：
10.1126/science.1190485
发表时间：
2010-07-23
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Wu H;Coskun V;Tao J;Xie W;Ge W;Yoshikawa K;Li E;Zhang Y;Sun YE
通讯作者：
Sun YE
影响因子：
4.8
作者：
Rauch, Tibor A.;Pfeifer, Gerd P.
通讯作者：
Pfeifer, Gerd P.
影响因子：
3.7
作者：
Globisch D;Münzel M;Müller M;Michalakis S;Wagner M;Koch S;Brückl T;Biel M;Carell T
通讯作者：
Carell T
影响因子：
46.9
作者：
通讯作者：
--