Knockdown of UCA1 attenuated the progression of alcoholic fatty disease by sponging miR-214
Knockdown of UCA1 attenuated the progression of alcoholic fatty disease by sponging miR-214
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UCA1 的敲低通过海绵 miR-214 减轻酒精性脂肪病的进展
DOI：
10.1007/s00335-022-09953-0
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发表时间：
2022-04
期刊：
影响因子：
2.5
通讯作者：
Linquan Wu
中科院分区：
文献类型：
--
作者：
Huali Xiang;Binfeng Tu;Ming Luo;Ping Hou;Jiakun Wang;Rongguiyi Zhang;Linquan Wu
Alcoholic fatty liver (AFL) is the initial manifestation of Alcoholic liver disease which can develop into alcoholic cirrhosis even extensive necrosis of liver cells, which induces liver failure finally. This study aims to focus on the role of long noncoding RNA UCA1 in AFL and further explored possible mechanism of this disease. We first downloaded GSE28619 to identify the expression of UCA1 in patients with AFL and use lncRNAs microarray to confirm UCA1 expression in serum of patients with AFL. Then we established ethanol-induced L02 cell model to mimic hepatocyte injury condition. By conducting qRT-PCR, we measured the expression of LncRNA UCA1 and miR-214 in serum of patients and ethanol-induced L02 cell. MTT assay, transwell migration, ELISA, qRT-PCR, and western blotting analysis were applied to evaluating the effect of UCA1 on ethanol-induced L02 cell. The bioinformatics analysis and the rescue experiment were devoted to the underlying mechanism. In this study, we first detected the expression of UCA1 was up-regulated in serum of patients with AFL and ethanol-induced L02 cells. And knockdown of UCA1 reversed the inhibiting effect of ethanol on the biological behavior of L02 cells including cell proliferation, migration, and apoptosis. Besides, lncRNA UCA1 regulated the expression of KLF5 by sponging miR-214. LncRNA UCA1 regulated the biological behavior of ethanol-induced L02 cells by sponging miR-214, which may provide novel therapeutic strategies for alcoholic fatty liver.
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影响因子：
3.4
作者：
Yang Yang-Yang;Zhenghao Zhao;N. Hou;Yulong Li;Xiaofei Wang;Fei Wu;R. Sun;Jia Han;Hongfei Sun;T. Song;Chen Huang;Y. Shao
通讯作者：
Yang Yang-Yang;Zhenghao Zhao;N. Hou;Yulong Li;Xiaofei Wang;Fei Wu;R. Sun;Jia Han;Hongfei Sun;T. Song;Chen Huang;Y. Shao
影响因子：
3.9
作者：
E. Smekalova;Y. Kotelevtsev;D. Leboeuf;E. Shcherbinina;Anna S. Fefilova;T. Zatsepin;V. Koteliansky
通讯作者：
E. Smekalova;Y. Kotelevtsev;D. Leboeuf;E. Shcherbinina;Anna S. Fefilova;T. Zatsepin;V. Koteliansky
LncRNA HOTAIR 通过增强 SP1 转录因子调节乙型肝炎病毒转录和复制
影响因子：
6
作者：
Ren, Fang;Ren, Ji-Hua;Yang, Xiao
通讯作者：
Yang, Xiao
DOI：
10.1152/ajpgi.00242.2017
发表时间：
2018-05-01
影响因子：
4.5
作者：
Huang, Xinli;Gao, Yun;Lu, Sen
通讯作者：
Lu, Sen
影响因子：
5.9
作者：
Vinolo MA;Rodrigues HG;Nachbar RT;Curi R
通讯作者：
Curi R