Enzymes degraded under high light maintain proteostasis by transcriptional regulation in Arabidopsis.
Enzymes degraded under high light maintain proteostasis by transcriptional regulation in Arabidopsis.
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DOI：
10.1073/pnas.2121362119
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发表时间：
2022-05-17
影响因子：
11.1
通讯作者：
中科院分区：
文献类型：
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作者：
光抑制强光胁迫导致植物体内蛋白质降解标记物的增加，蛋白水解酶和蛋白分解机制的转录上调，但大多数酶的蛋白质稳态在很大程度上是在强光下维持的，因此除了光系统损伤之外，我们对它的代谢后果知之甚少。我们开发了一种通过13C部分标记和详细的多肽质谱学来寻找强光响应的快速蛋白质周转事件的技术。这一分析揭示了光诱导的核编码基因的转录程序，超越了光系统II的调节，以取代植物中的关键蛋白质降解目标，并确保在高光胁迫下的蛋白稳定。在拟南芥中，光抑制强光胁迫导致蛋白质降解标记物的增加，以及蛋白水解酶和蛋白分解机制的转录上调，但蛋白稳定在很大程度上是维持的。我们发现，特定的分子伴侣、硝酸还原酶、甘油醛-3磷酸脱氢酶和磷酸甘油酸激酶以及其他参与氧化还原穿梭的质体酶、线粒体酶、过氧化物酶和细胞质酶的体内降解率显著增加。蛋白质降解率、mRNA水平和蛋白质丰度的联合分析表明，在降解率较高的核编码酶中，57%的酶也具有高光诱导的转录反应，以维持蛋白平衡。相比之下，降解率提高的叶绿体编码蛋白质显示转录丰度降低，必须通过其他过程保持蛋白质丰度。这一分析揭示了光诱导核编码基因的转录程序，超越了光系统II(PSII)D1亚基和PSII功能的调节，以取代植物中关键的蛋白质降解靶标，并确保高光胁迫下的蛋白稳定。
Photoinhibitory high light stress in plants leads to increases in markers of protein degradation and transcriptional up-regulation of proteases and proteolytic machinery, but protein homeostasis (proteostasis) of most enzymes is largely maintained under high light, so we know little about the metabolic consequences of it beyond photosystem damage. We developed a technique to look for rapid protein turnover events in response to high light through 13C partial labeling and detailed peptide mass spectrometry. This analysis reveals a light-induced transcriptional program for nuclear-encoded genes, beyond the regulation of photosystem II, to replace key protein degradation targets in plants and ensure proteostasis under high light stress. Photoinhibitory high light stress in Arabidopsis leads to increases in markers of protein degradation and transcriptional up-regulation of proteases and proteolytic machinery, but proteostasis is largely maintained. We find significant increases in the in vivo degradation rate for specific molecular chaperones, nitrate reductase, glyceraldehyde-3 phosphate dehydrogenase, and phosphoglycerate kinase and other plastid, mitochondrial, peroxisomal, and cytosolic enzymes involved in redox shuttles. Coupled analysis of protein degradation rates, mRNA levels, and protein abundance reveal that 57% of the nuclear-encoded enzymes with higher degradation rates also had high light–induced transcriptional responses to maintain proteostasis. In contrast, plastid-encoded proteins with enhanced degradation rates showed decreased transcript abundances and must maintain protein abundance by other processes. This analysis reveals a light-induced transcriptional program for nuclear-encoded genes, beyond the regulation of the photosystem II (PSII) D1 subunit and the function of PSII, to replace key protein degradation targets in plants and ensure proteostasis under high light stress.
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影响因子：
11.6
作者：
Bhuiyan, Nazmul H.;Friso, Giulia;van Wijk, Klaas J.
通讯作者：
van Wijk, Klaas J.
影响因子：
3.4
作者：
Deutsch, Eric W.;Mendoza, Luis;Shteynberg, David;Farrah, Terry;Lam, Henry;Tasman, Natalie;Sun, Zhi;Nilsson, Erik;Pratt, Brian;Prazen, Bryan;Eng, Jimmy K.;Martin, Daniel B.;Nesvizhskii, Alexey I.;Aebersold, Ruedi
通讯作者：
Aebersold, Ruedi
影响因子：
4.8
作者：
Collin, V;Issakidis-Bourguet, E;Miginiac-Maslow, M
通讯作者：
Miginiac-Maslow, M
影响因子：
64.8
作者：
Chatterjee, Abhishek;Abeydeera, N. Dinuka;Bale, Shridhar;Pai, Pei-Jing;Dorrestein, Pieter C.;Russell, David H.;Ealick, Steven E.;Begley, Tadhg P.
通讯作者：
Begley, Tadhg P.
DOI：
10.1073/pnas.2023348118
发表时间：
2021-03-30
影响因子：
11.1
作者：
Hanson AD;McCarty DR;Henry CS;Xian X;Joshi J;Patterson JA;García-García JD;Fleischmann SD;Tivendale ND;Millar AH
通讯作者：
Millar AH