Opposing activity changes in AMP deaminase and AMP-activated protein kinase in the hibernating ground squirrel.
Opposing activity changes in AMP deaminase and AMP-activated protein kinase in the hibernating ground squirrel.
复制标题
DOI：
10.1371/journal.pone.0123509
复制
发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Johnson RJ
中科院分区：
文献类型：
--
作者：
Lanaspa MA;Epperson LE;Li N;Cicerchi C;Garcia GE;Roncal-Jimenez CA;Trostel J;Jain S;Mant CT;Rivard CJ;Ishimoto T;Shimada M;Sanchez-Lozada LG;Nakagawa T;Jani A;Stenvinkel P;Martin SL;Johnson RJ
冬眠动物在夏季活动时会产生脂肪肝，并在冬季转换为脂肪氧化状态。我们假设这种转换可能是由AMP和相反效应的主导作用决定的：通过AMP脱氨酶（AMPD 2）（夏季）和AMP激活的蛋白激酶（AMPK）（冬季）的代谢。在一年中的不同时间，包括夏季和冬季冬眠的多个阶段，从13线地松鼠获得肝脏样品，并评估脂肪合成和β-脂肪酸氧化。脂肪代谢的变化与AMPD 2活性和肝内尿酸（AMPD 2的下游产物）的变化以及AMPK和肝内β-羟基丁酸（脂肪氧化的标志物）的变化相关。肝脏脂肪蓄积发生在夏季，与脂肪合成相关的酶（FAS、ACL和ACC）相对增加，脂肪氧化的限速酶烯酰CoA水合酶（ECH 1）和肉毒碱棕榈酰转移酶1A（CPT 1A）减少。在夏季，AMPD 2活性和肝内尿酸水平较高，肝脏AMPK活性较低。相反，AMPK和β-羟基丁酸的活性磷酸化形式在冬眠期间均增加。因此，AMPD 2和AMPK活性的变化与夏季-冬季周期中脂肪合成和脂肪氧化速率的变化相一致。这些数据阐明了AMPD 2代谢AMP的相反力量及其激活AMPK作为控制冬眠地松鼠肝脏中脂肪代谢的开关的可用性。
Hibernating animals develop fatty liver when active in summertime and undergo a switch to a fat oxidation state in the winter. We hypothesized that this switch might be determined by AMP and the dominance of opposing effects: metabolism through AMP deaminase (AMPD2) (summer) and activation of AMP-activated protein kinase (AMPK) (winter). Liver samples were obtained from 13-lined ground squirrels at different times during the year, including summer and multiples stages of winter hibernation, and fat synthesis and β-fatty acid oxidation were evaluated. Changes in fat metabolism were correlated with changes in AMPD2 activity and intrahepatic uric acid (downstream product of AMPD2), as well as changes in AMPK and intrahepatic β-hydroxybutyrate (a marker of fat oxidation). Hepatic fat accumulation occurred during the summer with relatively increased enzymes associated with fat synthesis (FAS, ACL and ACC) and decreased enoyl CoA hydratase (ECH1) and carnitine palmitoyltransferase 1A (CPT1A), rate limiting enzymes of fat oxidation. In summer, AMPD2 activity and intrahepatic uric acid levels were high and hepatic AMPK activity was low. In contrast, the active phosphorylated form of AMPK and β-hydroxybutyrate both increased during winter hibernation. Therefore, changes in AMPD2 and AMPK activity were paralleled with changes in fat synthesis and fat oxidation rates during the summer-winter cycle. These data illuminate the opposing forces of metabolism of AMP by AMPD2 and its availability to activate AMPK as a switch that governs fat metabolism in the liver of hibernating ground squirrel.
登录
查看更多内容
影响因子：
4.6
作者：
Epperson, L. Elaine;Karimpour-Fard, Anis;Martin, Sandra L.
通讯作者：
Martin, Sandra L.
DOI：
10.1016/j.cbpb.2005.08.010
发表时间：
2005-12-01
影响因子：
2.2
作者：
Horman, S;Hussain, N;Rider, MH
通讯作者：
Rider, MH
影响因子：
4.2
作者：
Keesey, RE;Hirvonen, MD
通讯作者：
Hirvonen, MD
DOI：
10.1152/ajpregu.90795.2008
发表时间：
2009-02-01
影响因子：
2.8
作者：
Andrews, Matthew T.;Russeth, Kevin P.;Henry, Pierre-Gilles
通讯作者：
Henry, Pierre-Gilles
影响因子：
--
作者：
FLORANT, GL;LAWRENCE, AK;BAUMAN, WA
通讯作者：
BAUMAN, WA