10 Gy total body irradiation increases risk of coronary sclerosis, degeneration of heart structure and function in a rat model.
10 Gy total body irradiation increases risk of coronary sclerosis, degeneration of heart structure and function in a rat model.
复制标题
DOI：
10.3109/09553000903264473
复制
发表时间：
2009-12
影响因子：
2.6
通讯作者：
Moulder JE
中科院分区：
文献类型：
--
作者：
Baker JE;Fish BL;Su J;Haworth ST;Strande JL;Komorowski RA;Migrino RQ;Doppalapudi A;Harmann L;Allen Li X;Hopewell JW;Moulder JE
确定10戈伊全身照射（TBI）或局部胸部照射（与放射性恐怖威胁相关的剂量）对脂质和肝脏特征、冠状动脉微血管和心室功能的影响。WAG/RijCmcr大鼠接受10戈伊TBI后进行骨髓移植或10戈伊局部胸部照射。与之匹配的未受辐射的大鼠作为对照。比较血脂和肝酶，冠状动脉血管形态，一氧化氮合酶（NOS）亚型，蛋白酶激活受体（PAR）-1表达和纤维蛋白原水平。二维应变超声心动图评估心脏的整体径向和周向应变。TBI导致总胆固醇和低密度脂蛋白（LDL）胆固醇持续升高（分别为190±8 vs. 58±6; 82±8 vs. 13±3 mg/dL）。冠状小动脉密度降低32%。组织学显示一些血管完全堵塞，而心肌细胞保持正常。TBI导致细胞动脉周围纤维化，而对照心脏具有对称的穿透血管，胶原蛋白和成纤维细胞较少。TBI 120天后，内皮型NOS和诱导型NOS蛋白分别下降32±4%和28±3%，纤维蛋白原和PAR-1蛋白分别升高21±4%和35±5%。与对照组相比，TBI降低了径向应变（19±8 vs. 46±7%）和周向应变（-8 3 vs. −15±3%）。仅胸部照射在相同的时间范围内没有产生变化。10戈伊的TBI（与放射性恐怖威胁相关的剂量）使血脂水平恶化，冠状动脉微血管受损，内皮生理学和心肌力学改变。这些变化在胸部局部照射时不明显。非胸部循环因素可能会促进辐射引起的心脏损伤。
To determine the impact of 10 Gy total body irradiation (TBI) or local thorax irradiation, a dose relevant to a radiological terrorist threat, on lipid and liver profile, coronary microvasculature and ventricular function. WAG/RijCmcr rats received 10 Gy TBI followed by bone marrow transplantation, or 10 Gy local thorax irradiation. Age-matched, non-irradiated rats served as controls. The lipid profile and liver enzymes, coronary vessel morphology, nitric oxide synthase (NOS) isoforms, protease activated receptor (PAR)-1 expression and fibrinogen levels were compared. Two dimensional strain echocardiography assessed global radial and circumferential strain on the heart. TBI resulted in a sustained increase in total and low density lipoprotein (LDL) cholesterol (190±8 vs. 58±6; 82±8 vs. 13±3 mg/dL, respectively). The density of small coronary arterioles was decreased by 32%. Histology revealed complete blockage of some vessels while cardiomyocytes remained normal. TBI resulted in cellular peri-arterial fibrosis whereas control hearts had symmetrical penetrating vessels with less collagen and fibroblasts. TBI resulted in a 32±4% and 28±3% decrease in endothelial NOS and inducible NOS protein respectively, and a 21±4% and 35±5% increase in fibrinogen and PAR-1 protein respectively, after 120 days. TBI reduced radial strain (19±8 vs. 46±7%) and circumferential strain (-8±3 vs. −15±3%) compared to controls. Thorax-only irradiation produced no changes over the same time frame. TBI with 10 Gy, a dose relevant to radiological terrorist threats, worsened lipid profile, injured coronary microvasculature, altered endothelial physiology and myocardial mechanics. These changes were not manifest with local thorax irradiation. Non-thoracic circulating factors may be promoting radiation-induced injury to the heart.
登录
查看更多内容
影响因子：
2.6
作者：
Datta, PK;Moulder, JE;Lianos, EA
通讯作者：
Lianos, EA
影响因子：
4.8
作者：
Eames, GM;Crosson, J;Neglia, JP
通讯作者：
Neglia, JP
影响因子：
7.8
作者：
Cohen, Eric P.;Fish, Brian L.;Moulder, John E.
通讯作者：
Moulder, John E.
影响因子：
10.8
作者：
Baker, John E.;Su, Jidong;Gross, Garrett J.
通讯作者：
Gross, Garrett J.
影响因子：
3.4
作者：
Coleman, C. Norman;Blakely, William F.;Wong, Rosemary S. L.
通讯作者：
Wong, Rosemary S. L.