Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017.
Field Deployment of a Mobile Biosafety Laboratory Reveals the Co-Circulation of Dengue Viruses Serotype 1 and Serotype 2 in Louga City, Senegal, 2017.
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DOI：
10.1155/2021/8817987
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发表时间：
2021
影响因子：
2.2
通讯作者：
Faye O
中科院分区：
文献类型：
--
作者：
Dieng I;Diarra M;Diagne MM;Faye M;Dior Ndione MH;Ba Y;Diop M;Ndiaye EH;Marinho de Andrade Zanotto P;Diop B;Ndiaye M;Bousso A;Dia N;Diallo M;Barry A;Fall G;Loucoubar C;Sall AA;Faye O;Faye O
登革病毒（DENV）是世界范围内最普遍的虫媒病毒威胁。这种病毒属于黄病毒科黄病毒属，引起广泛的临床表现，从无症状或轻度发热性疾病（登革热）到危及生命的感染（严重登革热）。自1970年以来，塞内加尔发生了许多零星病例和疫情。尽管如此，本文描述了2017年9月5日至2017年12月17日期间通过部署移动的生物安全实验室（MBS-Lab）对疑似登革热病例进行的实地调查。总共收集了960份人血清，并通过实时RT-PCR在现场检测病毒RNA的存在。同时进行血清学分型、E基因全序列测定和系统发育分析。在960例疑似病例中，131例为确诊登革热病例。大多数确诊病例来自Louga社区。血清分型显示两种血清型，登革热1型（100/104; 96，15%）和登革热2型（04/104; 3，84%）。对获得的序列的系统发育分析表明，登革热1型毒株与2013年新加坡（亚洲）爆发的毒株（KX 380803.1）密切相关，并与2016年布基纳法索登革热爆发的毒株（KY62776.1）密切相关的登革热2型毒株共传播。我们的研究结果表明，在一次暴发中，两种登革热病毒血清型在短时间内共同传播。这种共同传播强调了改善监测的必要性，以便通过抗体依赖性增强（ADE）预防未来潜在的严重登革热病例。有趣的是，它还证明了MBS-Lab在需要时快速应对疫情的可靠性和实用性，这使得早期病例管理成为可能。
Dengue virus (DENV) is the most prevalent arboviral threat worldwide. This virus belonging to genus Flavivirus, Flaviviridae family, is responsible for a wide spectrum of clinical manifestations, ranging from asymptomatic or mild febrile illness (dengue fever) to life-threatening infections (severe dengue). Many sporadic cases and outbreaks have occurred in Senegal since 1970. Nevertheless, this article describes a field investigation of suspected dengue cases, between 05 September 2017 and 17 December 2017 made possible by the deployment of a Mobile Biosafety Laboratory (MBS-Lab). Overall, 960 human sera were collected and tested in the field for the presence of viral RNA by real-time RT-PCR. Serotyping, sequencing of complete E gene, and phylogenetic analysis were also performed. Out of 960 suspected cases, 131 were confirmed dengue cases. The majority of confirmed cases were from Louga community. Serotyping revealed two serotypes, Dengue 1 (100/104; 96, 15%) and Dengue 2 (04/104; 3, 84%). Phylogenetic analysis of the sequences obtained indicated that the Dengue 1 strain was closely related to strains isolated, respectively, in Singapore (Asia) in 2013 (KX380803.1) outbreak and it cocirculated with a Dengue 2 strain closely related to strains from a Burkina Faso dengue outbreak in 2016 (KY62776.1). Our results showed the co-circulation of two dengue virus serotypes during a single outbreak in a short time period. This co-circulation highlighted the need to improve surveillance in order to prevent future potential severe dengue cases through antibody-dependent enhancement (ADE). Interestingly, it also proved the reliability and usefulness of the MBS-Lab for expedient outbreak response at the point of need, which allows early cases management.
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DOI：
10.3390/v3091562
发表时间：
2011-09
期刊：
Viruses
影响因子：
--
作者：
Chen R;Vasilakis N
通讯作者：
Vasilakis N
中国广东省自然群体中具有 3 个重组区的 1 型重组登革热病毒的鉴定。
影响因子：
2.7
作者：
Chen SP;Yu M;Jiang T;Deng YQ;Qin CF;Han JF;Qin ED
通讯作者：
Qin ED
DOI：
10.1128/genomea.00209-17
发表时间：
2017-04-01
期刊：
GENOME ANNOUNCEMENTS
影响因子：
--
作者：
Baronti, Cecile;Piorkowski, Geraldine;Nougairede, Antoine
通讯作者：
Nougairede, Antoine
影响因子：
10.7
作者：
Nguyen LT;Schmidt HA;von Haeseler A;Minh BQ
通讯作者：
Minh BQ
影响因子：
3.7
作者：
Dia N;Diene Sarr F;Thiam D;Faye Sarr T;Espié E;OmarBa I;Coly M;Niang M;Richard V;4S Network Group
通讯作者：
4S Network Group