The recent escalation in strength of pyrethroid resistance in Anopheles coluzzi in West Africa is linked to increased expression of multiple gene families.
The recent escalation in strength of pyrethroid resistance in Anopheles coluzzi in West Africa is linked to increased expression of multiple gene families.
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DOI：
10.1186/s12864-015-1342-6
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发表时间：
2015-03-01
期刊：
影响因子：
4.4
通讯作者：
Jones CM
中科院分区：
文献类型：
--
作者：
Toé KH;N'Falé S;Dabiré RK;Ranson H;Jones CM
自2011年以来，布基纳法索主要疟疾蚊子----Anopheles coluzzi----对拟除虫菊酯的抗药性水平已经上升到如此程度，以至于目前全国各地使用的长效驱虫蚊帐都无法杀死当地的蚊媒。我们使用2011年和2012年进行的两项独立的全基因组微阵列研究调查了这种观察到的增加是否与野外捕获的按蚊coluzzi的转录变化相关。2011年和2012年从布基纳法索西南部收集了蚊子，并使用全基因组微阵列将接触或未接触杀虫剂的昆虫与实验室易感群体进行了比较。使用严格的过滤过程，我们确定了136个基因，包括充分研究的解毒酶（p450单加氧酶和酯酶）和非解毒基因（例如细胞转运蛋白和表皮成分），与拟除虫菊酯抗性相关，其基础表达水平在研究的时间范围内增加。使用来自两个研究中心的样本通过qPCR验证了这些样本的子集，这些样本在3年内收集，并且每年观察到表达显著增加。我们假设这些基因是导致这种快速增加的耐药表型在AN。coluzzi对敲低耐药（kdr）突变（L1014 S、L1014 F和N1575 Y）的综合分析显示，大多数耐药表型不能通过靶位点修饰来解释。我们的数据表明，最近在布基纳法索西南部观察到的拟除虫菊酯耐药性迅速增加与这里描述的基因表达谱有关。超过三分之一的这些候选物也在多个拟除虫菊酯抗性的An群体中过表达。来自邻国科特迪瓦的coluzzi。这套分子标记可用于跟踪席卷西非的极端拟除虫菊酯抗性表型的传播，并确定该性状的功能基础。本文的在线版本（doi：10.1186/s12864-015-1342-6）包含补充材料，可供授权用户使用。
Since 2011, the level of pyrethroid resistance in the major malaria mosquito, Anopheles coluzzi, has increased to such an extent in Burkina Faso that none of the long lasting insecticide treated nets (LLINs) currently in use throughout the country kill the local mosquito vectors. We investigated whether this observed increase was associated with transcriptional changes in field-caught Anopheles coluzzi using two independent whole-genome microarray studies, performed in 2011 and 2012. Mosquitoes were collected from south-west Burkina Faso in 2011 and 2012 and insecticide exposed or non-exposed insects were compared to laboratory susceptible colonies using whole-genome microarrays. Using a stringent filtering process we identified 136 genes, including the well-studied detoxification enzymes (p450 monoxygenases and esterases) and non-detoxification genes (e.g. cell transporters and cuticular components), associated with pyrethroid resistance, whose basal expression level increased during the timeframe of the study. A subset of these were validated by qPCR using samples from two study sites, collected over 3 years and marked increases in expression were observed each year. We hypothesise that these genes are contributing to this rapidly increasing resistance phenotype in An. coluzzi. A comprehensive analysis of the knockdown resistance (kdr) mutations (L1014S, L1014F and N1575Y) revealed that the majority of the resistance phenotype is not explained by target-site modifications. Our data indicate that the recent and rapid increase in pyrethroid resistance observed in south-west Burkina Faso is associated with gene expression profiles described here. Over a third of these candidates are also overexpressed in multiple pyrethroid resistant populations of An. coluzzi from neighbouring Côte d’Ivoire. This suite of molecular markers can be used to track the spread of the extreme pyrethroid resistance phenotype that is sweeping through West Africa and to determine the functional basis of this trait. The online version of this article (doi:10.1186/s12864-015-1342-6) contains supplementary material, which is available to authorized users.
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影响因子：
11.8
作者：
Edi CV;Koudou BG;Jones CM;Weetman D;Ranson H
通讯作者：
Ranson H
影响因子：
3.9
作者：
Gong, MQ;Shen, B;Zhu, CL
通讯作者：
Zhu, CL
现场捕获的耐氯菊酯抗蚊子冈比亚过表达CYP6P3，一种代谢拟除虫菊酯的P450。
影响因子：
4.5
作者：
Mueller, Pie;Warr, Emma;Stevenson, Bradley J.;Pignatelli, Patricia M.;Morgan, John C.;Steven, Andrew;Yawson, Alexander E.;Mitchell, Sara N.;Ranson, Hilary;Hemingway, Janet;Paine, Mark J. I.;Donnelly, Martin J.
通讯作者：
Donnelly, Martin J.
影响因子：
3
作者：
Bass, Chris;Nikou, Dimitra;Donnelly, Martin J.;Williamson, Martin S.;Ranson, Hilary;Ball, Amanda;Vontas, John;Field, Linda M.
通讯作者：
Field, Linda M.
影响因子：
12.3
作者：
Cui X;Churchill GA
通讯作者：
Churchill GA