Recurrent Co-Option and Recombination of Cytokine and Three Finger Proteins in Multiple Reproductive Tissues Throughout Salamander Evolution.
Recurrent Co-Option and Recombination of Cytokine and Three Finger Proteins in Multiple Reproductive Tissues Throughout Salamander Evolution.
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DOI：
10.3389/fcell.2022.828947
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发表时间：
2022
影响因子：
5.5
通讯作者：
Searle BC
中科院分区：
文献类型：
--
作者：
Wilburn DB;Kunkel CL;Feldhoff RC;Feldhoff PW;Searle BC
生殖蛋白以无与伦比的速度进化，导致不同分类支系的配子之间分子组成和生化功能都存在巨大差异。到目前为止，两栖配子的蛋白质组组成在很大程度上是一个分子之谜，特别是对于蜥蜴和蝾螈来说，它们几乎没有基因组规模的资源。在这项研究中，我们首次提供了两个物种(Plethodon shermani和Desmognatus Ocoee)的配子的详细分子特征，这两个物种是繁殖行为的模式。对这两个物种的睾丸和卵巢进行了长时间的PacBio转录组测序，发现许多脊椎动物配子共有的基因具有性别特异性表达，包括与非洲爪哇卵母细胞的卵壳基因相似的表达谱。与卵母细胞基因的广泛保守性不同，主要的睾丸转录包括火蜥蜴特有的求偶信息素(PRF、PMF和SPF)的副组，这些信息素被质谱仪蛋白质组学证实为主要的精子蛋白。精子特异的PMF和SPF蛋白可能分别是沙门氏疟原虫和大口石龙虾中最丰富的分泌蛋白。相反，精子PRF缺乏信号肽，可能在细胞质中表达。PRF信息素基因通过重复基因复制精子PRF基因，通过与PMF基因重组回收信号肽，多次独立进化。对求偶信息素及其精子配对的系统发育分析支持，每个蛋白质家族在1700万至3.6亿年前的不同进化时间点为这两种生殖环境进化。我们对多齿目生殖组织的系统发育、转录组和蛋白质组的联合分析支持，TFP和细胞因子样蛋白的反复选择和重组已经成为火蜥蜴进化和繁殖的新驱动力。
Reproductive proteins evolve at unparalleled rates, resulting in tremendous diversity of both molecular composition and biochemical function between gametes of different taxonomic clades. To date, the proteomic composition of amphibian gametes is largely a molecular mystery, particularly for Urodeles (salamanders and newts) for which few genomic-scale resources exist. In this study, we provide the first detailed molecular characterization of gametes from two salamander species (Plethodon shermani and Desmognathus ocoee) that are models of reproductive behavior. Long-read PacBio transcriptome sequencing of testis and ovary of both species revealed sex-specific expression of many genes common to vertebrate gametes, including a similar expression profile to the egg coat genes of Xenopus oocytes. In contrast to broad conservation of oocyte genes, major testis transcripts included paralogs of salamander-specific courtship pheromones (PRF, PMF, and SPF) that were confirmed as major sperm proteins by mass spectrometry proteomics. Sperm-specific paralogs of PMF and SPF are likely the most abundant secreted proteins in P. shermani and D. ocoee, respectively. In contrast, sperm PRF lacks a signal peptide and may be expressed in cytoplasm. PRF pheromone genes evolved independently multiple times by repeated gene duplication of sperm PRF genes with signal peptides recovered through recombination with PMF genes. Phylogenetic analysis of courtship pheromones and their sperm paralogs support that each protein family evolved for these two reproductive contexts at distinct evolutionary time points between 17 and 360 million years ago. Our combined phylogenetic, transcriptomic and proteomic analyses of plethodontid reproductive tissues support that the recurrent co-option and recombination of TFPs and cytokine-like proteins have been a novel driving force throughout salamander evolution and reproduction.
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DOI：
10.1073/pnas.2108777118
发表时间：
2021-09-28
影响因子：
11.1
作者：
Fujihara Y;Herberg S;Blaha A;Panser K;Kobayashi K;Larasati T;Novatchkova M;Theussl HC;Olszanska O;Ikawa M;Pauli A
通讯作者：
Pauli A
影响因子：
3.9
作者：
Herrboldt, Madison A.;Steffen, Michael A.;Bonett, Ronald M.
通讯作者：
Bonett, Ronald M.
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
5.4
作者：
Galat, Andrzej;Gross, Gregory;Menez, Andre
通讯作者：
Menez, Andre
影响因子：
1.1
作者：
Herrick, John;Sclavi, Bianca
通讯作者：
Sclavi, Bianca