Arabidopsis FHY3/CPD45 regulates far-red light signaling and chloroplast division in parallel.
Arabidopsis FHY3/CPD45 regulates far-red light signaling and chloroplast division in parallel.
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DOI：
10.1038/srep09612
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发表时间：
2015-04-15
影响因子：
4.6
通讯作者：
Gao H
中科院分区：
文献类型：
--
作者：
Chang N;Gao Y;Zhao L;Liu X;Gao H
CPD 45（chloroplast division 45），又称FHY 3（far-red elongated hypocotyl 3），是拟南芥远红光信号转导途径中的一个关键因子。我们先前表明FHY 3/CPD 45也调节叶绿体分裂。由于光也是叶绿体发育和分裂的调节剂，我们试图阐明远红光信号和叶绿体分裂途径之间的关系。我们发现叶绿体分裂突变体arc 5 -3在远红光感应中没有缺陷，并且组成型过表达ARC 5拯救了叶绿体分裂缺陷，但没有拯救cpd 45在远红光信号传导中的缺陷。在远红光信号传导中有缺陷的FHY 1表现出正常的叶绿体分裂。FHY 1的组成性过表达挽救了cpd 45的远红光信号缺陷，但没有挽救叶绿体分裂缺陷。此外，ARC 5和FHY 1的表达分别在fhy 1和arc 5 -3中不受影响。基于这些结果，我们提出FHY 3/CPD 45通过独立激活FHY 1和ARC 5的表达来平行调节远红光信号和叶绿体分裂。这项工作展示了如何在基因调控网络中的不同途径之间的关系可以探索。
CPD45 (chloroplast division45),which is also known as FHY3 (far-red elongated hypocotyl3), is a key factor in the far-red light signaling pathway in Arabidopsis. We previously showed that FHY3/CPD45 also regulates chloroplast division. Because light is also a regulator of chloroplast development and division, we sought to clarify the relationship between far-red light signaling and chloroplast division pathways. We found that the chloroplast division mutant arc5-3 had no defect in far-red light sensing, and that constitutive overexpression of ARC5 rescued the chloroplast division defect, but not the defect in far-red light signaling, of cpd45. fhy1, which is defective in far-red light signaling, exhibited normal chloroplast division. Constitutive overexpression of FHY1 rescued the far-red light signaling defect, but not the chloroplast division defect, of cpd45. Moreover, ARC5 and FHY1 expression were not affected in fhy1 and arc5-3, respectively. Based on these results, we propose that FHY3/CPD45 regulates far-red light signaling and chloroplast division in parallel by activating the expression of FHY1 and ARC5 independently. This work demonstrates how relationships between different pathways in a gene regulatory network can be explored.
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影响因子：
7.4
作者：
Jeong, WJ;Park, YI;Liu, JR
通讯作者：
Liu, JR
影响因子：
4.5
作者：
Genoud, Thierry;Schweizer, Fabian;Tscheuschler, Anke;Debrieux, Dimitry;Casal, Jorge J.;Schaefer, Eberhard;Hiltbrunner, Andreas;Fankhauser, Christian
通讯作者：
Fankhauser, Christian
影响因子：
6.9
作者：
Königer M;Delamaide JA;Marlow ED;Harris GC
通讯作者：
Harris GC
DOI：
10.1073/pnas.1318511111
发表时间：
2014-01-21
影响因子：
11.1
作者：
Bates, Philip D.;Johnson, Sean R.;Browse, John
通讯作者：
Browse, John
影响因子：
11.6
作者：
Ouyang, Xinhao;Li, Jigang;Deng, Xing Wang
通讯作者：
Deng, Xing Wang