Surface modification of Ti45Nb alloy by immobilization of RGD peptide via self assembled monolayer
Surface modification of Ti45Nb alloy by immobilization of RGD peptide via self assembled monolayer
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自组装单层固定RGD肽对Ti45Nb合金进行表面修饰
DOI：
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
C. Sukenik
中科院分区：
文献类型：
--
作者：
G. Zorn;I. Gotman;E. Gutmanas;Racheli Adadi;C. Sukenik
目前正在考虑将一种具有低弹性模量和优异耐腐蚀性的新型低模量β Ti-Nb合金作为外科植入物材料。如果可以控制这些材料的表面结构和表面化学，则可以显著增强这些材料的有用性。这种控制是通过将基于11-氯乙酰基-1-十一烷基膦酸（CAUDPA）的自组装单层（SAM）附着到表面并将肽固定到单层来实现的。在两种不同的起飞角度下，利用傅里叶变换红外光谱（FTIR）和X射线光电子能谱（XPS）对SAM进行了表征。CAUDPA分子共价键合在基板上的配置，其中膦酸基团转向Ti45 Nb，而乙酰氯端基尾部转向最顶部的表面。在这种配置中，通过氯化物和生物分子之间的交换，顺序原位反应是可能的。这种生物分子是精氨酸-甘氨酸-天冬氨酸-半胱氨酸，RGDC，存在于细胞外基质的许多分子中的小氨基酸序列。初步的体外细胞培养结果显示，肽固定后成骨细胞对Ti45 Nb的反应有所改善。
A new low modulus β Ti-Nb alloy with low elastic modulus and excellent corrosion resistance is currently under consideration as a surgical implant material. The usefulness of such materials can be dramatically enhanced if their surface structure and surface chemistry can be controlled. This control is achieved by attaching a self assembled monolayer (SAM) based on 11-chloroacetyl-1-undecylphosphonic acid, CAUDPA, to the surface and immobilization of a peptide to the monolayer. The SAM is characterized by Fourier Transform Infrared Spectroscopy (FTIR) and X-ray Photoelectron Spectroscopy (XPS) at two different takeoff angles. The CAUDPA molecules were covalently bonded on the substrate in a configuration in which the phosphonic group turns toward the Ti45Nb while the acetyl chloride end group tail turns to the topmost surface. In such configuration sequential in situ reaction is possible by exchange between the chloride and a biological molecule. Such biological molecule is the arginine-glycine-aspartic acid-cysteine, RGDC, small amino acid sequence present in many molecules of the extracellular matrix. Preliminary cell culture in-vitro result shows an improvement of the response of osteoblast cells to Ti45Nb after the peptide immobilization.
影响因子：
56.9
作者：
RUOSLAHTI, E;PIERSCHBACHER, MD
通讯作者：
PIERSCHBACHER, MD
影响因子：
56.9
作者：
GRISTINA, AG
通讯作者：
GRISTINA, AG