Neurogenesis and Commitment of Corticospinal Neurons inreeler
Neurogenesis and Commitment of Corticospinal Neurons inreeler
复制标题
卷轴中皮质脊髓神经元的神经发生和承诺
作者：
F. Polleux;C. Dehay;H. Kennedy
在纯合子（而非杂合子）reelermutant中，神经元迁移的中断导致皮质环境的重大扰动，进而可以改变(1)神经元命运的规范和(2)皮质前体的增殖动力学。为了研究这些问题，在皮质神经发生期间注射氚化胸腺嘧啶，并在出生后在脊髓注射逆行示踪剂，以准确测量杂合子和纯合子突变体皮质脊髓神经元的神经发生。纯合卷轴显示：(1)皮质脊髓神经元产生的区域特异性时间表严格守恒；(2)出生日期合适的神经元向脊髓投射的概率更高；(3)在皮质生成的早期阶段，神经元生成速率降低，随后在后期阶段神经元生成速率增加；(4)神经元产生率的这些变化至少部分归因于分化分裂比例的变化。综上所述，我们的研究结果表明，在发育中的皮层中，神经发生和特定神经元表型部分由迁移后隔室控制。另一方面，无论是区域身份还是层特异性神经元表型的产生年表似乎都不依赖于细胞环境的完整性。
In the homozygous (but not the heterozygous) reelermutant, disruption of neuron migration leads to a major perturbation of the cortical environment that in turn could modify (1) the specification of neuronal fate and (2) the proliferation dynamics of cortical precursors. To investigate these issues, tritiated thymidine injections during cortical neurogenesis were coupled with postnatal injections of a retrograde tracer in the spinal cord to accurately measure the neurogenesis of corticospinal neurons in the heterozygous and homozygous mutant. The homozygous reeler shows (1) strict conservation of area-specific timetables of corticospinal neuron generation; (2) neurons with the appropriate birthdates show an enhanced probability of projecting to the spinal cord; (3) during early stages of corticogenesis, there is a reduced rate of neuron production followed at later stages by an increased rate of neuron production; and (4) these changes in the rate of neuron production were shown to be at least partially attributable to changes in the proportions of differentiative divisions. Taken together, our results show that in the developing cortex, the neurogenesis and specification of a given neuronal phenotype are partially controlled by the postmigratory compartment. On the other hand, neither areal identity nor the chronology of production of layer-specific neuronal phenotype seems to depend on the integrity of the cellular environment.
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DOI：
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DOI：
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发表时间：
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影响因子：
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DOI：
10.1073/pnas.91.1.375
发表时间：
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影响因子：
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作者：
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