De novo transcriptome analysis of petal senescence in Gardenia jasminoides Ellis.
De novo transcriptome analysis of petal senescence in Gardenia jasminoides Ellis.
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DOI：
10.1186/1471-2164-15-554
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发表时间：
2014-07-04
期刊：
影响因子：
4.4
通讯作者：
Kalaitzis P
中科院分区：
文献类型：
--
作者：
Tsanakas GF;Manioudaki ME;Economou AS;Kalaitzis P
乙烯不敏感物种的花瓣衰老尚未得到彻底的研究，而很少有人知道的时间和组织特异性表达模式的转录因子（TF）在这个发展过程中。而对观赏盆栽植物栀子（Gardenia jasminoides）的衰老研究更是少之又少，栀子是一种非跃变型花卉，具有重要的商业价值。我们启动了从头转录组研究，调查花瓣衰老的四个发育阶段的栀子花考虑到可见的衰老症状出现在开花后4天内。转录组测序的从头组装产生102，263个重叠群，平均长度为360个核苷酸，产生57，503个单基因。这些被进一步聚类为20，970个集群和36，533个单体。连续发育阶段的比较导致180个共同的，差异表达的unigenes。还鉴定了大量简单序列重复，其包含大量二核苷酸和三核苷酸。差异表达的TF的主要家族包括AP 2/EREBP、WRKY和bHLH。当花衰老的症状变得可见时，有81个差异表达的TF，其中最普遍的是具有19个单基因的WRKY家族。在乙烯不敏感植物的花瓣衰老过程中，尚未发现其它WRKY转录因子。大量的差异表达的基因被确定在可见的衰老症状的开始相比，开放的花阶段，表明一个显着的转变，可能是协调上调和/或下调TF的表达谱。利用qRT-PCR验证了属于WRKY、bHLH和乙烯传感途径的TF家族的16个基因的表达。这种从头转录组分析导致识别具有特定时间表达模式的TF，例如两个WRKYs和一个bHLH，其可能发挥衰老进展调节剂的作用。需要进一步研究它们在栀子花中的作用，以开发延缓花瓣衰老的工具。本文的在线版本（doi：10.1186/1471-2164-15-554）包含补充材料，可供授权用户使用。
The petal senescence of ethylene insensitive species has not been investigated thoroughly while little is known about the temporal and tissue specific expression patterns of transcription factors (TFs) in this developmental process. Even less is known on flower senescence of the ornamental pot plant Gardenia jasminoides, a non climacteric flower with significant commercial value. We initiated a de novo transcriptome study to investigate the petal senescence in four developmental stages of cut gardenia flowers considering that the visible symptoms of senescence appear within 4 days of flower opening. De novo assembly of transcriptome sequencing resulted in 102,263 contigs with mean length of 360 nucleotides that generated 57,503 unigenes. These were further clustered into 20,970 clusters and 36,533 singletons. The comparison of the consecutive developmental stages resulted in 180 common, differentially expressed unigenes. A large number of Simple Sequence Repeats were also identified comprising a large number of dinucleotides and trinucleotides. The prevailing families of differentially expressed TFs comprise the AP2/EREBP, WRKY and the bHLH. There are 81 differentially expressed TFs when the symptoms of flower senescence become visible with the most prevailing being the WRKY family with 19 unigenes. No other WRKY TFs had been identified up to now in petal senescence of ethylene insensitive species. A large number of differentially expressed genes were identified at the initiation of visible symptoms of senescence compared to the open flower stage indicating a significant shift in the expression profiles which might be coordinated by up-regulated and/or down-regulated TFs. The expression of 16 genes that belong to the TF families of WRKY, bHLH and the ethylene sensing pathway was validated using qRT – PCR. This de novo transcriptome analysis resulted in the identification of TFs with specific temporal expression patterns such as two WRKYs and one bHLH, which might play the role of senescence progression regulators. Further research is required to investigate their role in gardenia flowers in order to develop tools to delay petal senescence. The online version of this article (doi:10.1186/1471-2164-15-554) contains supplementary material, which is available to authorized users.
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