Molecular characterization of seven genes encoding ethylene-responsive transcriptional factors during plum fruit development and ripening.
Molecular characterization of seven genes encoding ethylene-responsive transcriptional factors during plum fruit development and ripening.
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DOI：
10.1093/jxb/ern354
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发表时间：
2009
影响因子：
6.9
通讯作者：
Jayasankar S
中科院分区：
文献类型：
--
作者：
El-Sharkawy I;Sherif S;Mila I;Bouzayen M;Jayasankar S
从两个日本李子 (Prunus salicina L.) 品种“Early Golden”(EG) 和“Shiro”(SH) 中克隆了 7 个 ERF cDNA。根据序列特征，这些 Ps-ERF 可分为四个已知 ERF 家族中的三个。他们预测的氨基酸序列与其他植物物种的 ERF 相似。使用共聚焦显微镜对两种 Ps-ERF 蛋白（Ps-ERF1a 和 -1b）进行功能性核定位信号分析。对两个李子品种的 Ps-ERF mRNA 表达分析进行了研究，以确定该基因家族在果实发育和成熟中的作用。七个 Ps-ERF 在花和果实发育的各个阶段表现出差异表达模式和水平。 Ps-ERF 积累的多样性很大程度上是由于它们对乙烯产生水平的反应不同。然而，其他植物激素，如细胞分裂素和生长素，在各个发育阶段强烈积累，也会影响 Ps-ERF 的表达。研究了植物激素、赤霉素、细胞分裂素、生长素和乙烯在调节不同 Ps-ERF 转录本中的作用。提出了一个模型，其中植物激素生长素在启动和确定日本李子成熟的日期和速度方面所起的作用与乙烯的作用一样重要。
Seven ERF cDNAs were cloned from two Japanese plum (Prunus salicina L.) cultivars, ‘Early Golden’ (EG) and ‘Shiro’ (SH). Based on the sequence characterization, these Ps-ERFs could be classified into three of the four known ERF families. Their predicted amino acid sequences exhibited similarities to ERFs from other plant species. Functional nuclear localization signal analyses of two Ps-ERF proteins (Ps-ERF1a and -1b) were carried out using confocal microscopy. Expression analyses of Ps-ERF mRNAs were studied in the two plum cultivars in order to determine the role of this gene family in fruit development and ripening. The seven Ps-ERFs displayed differential expression pattern and levels throughout the various stages of flower and fruit development. The diversity in Ps-ERFs accumulation was largely due to the differences in their responses to the levels of ethylene production. However, other plant hormones such as cytokinin and auxin, which accumulate strongly throughout the various developmental stages, also influence the Ps-ERFs expression. The effect of the plant hormones, gibberellin, cytokinin, auxin, and ethylene in regulating the different Ps-ERF transcripts was investigated. A model was proposed in which the role played by the plant hormone auxin is as important as that of ethylene in initiating and determining the date and rate of ripening in Japanese plums.
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四个日本李栽培品种的成熟行为和对丙烯的反应
影响因子：
7
作者：
Abdi, N;Holford, P;Mizrahi, Y
通讯作者：
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DOI：
10.1073/pnas.94.11.5961
发表时间：
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影响因子：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
6.9
作者：
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影响因子：
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作者：
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