Site-Specific N-Glycan Characterization of Grass Carp Serum IgM.
Site-Specific N-Glycan Characterization of Grass Carp Serum IgM.
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草鱼血清 IgM 的位点特异性 N-聚糖表征
DOI：
10.3389/fimmu.2018.02645
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发表时间：
2018
影响因子：
7.3
通讯作者：
Geng H
中科院分区：
文献类型：
--
作者：
Su YL;Wang B;Hu MD;Cui ZW;Wan J;Bai H;Yang Q;Cui YF;Wan CH;Xiong L;Zhang YA;Geng H
免疫球蛋白M（IgM）是硬骨鱼体内的主要抗体，在体液适应性免疫中起重要作用。在高等脊椎动物中，IgM上呈现的N-连接碳水化合物已被充分记录，但关于硬骨鱼IgM中的位点特异性N-聚糖特征知之甚少。为了表征这些位点特异性的N-聚糖，我们进行了草鱼血清IgM的每个糖基化位点的N-聚糖的第一次研究。在四个糖基化位点中，Asn-262、Asn-303和Asn-426残基被有效糖基化，而C-末端尾片段的Asn-565残基未被完全占据。与单体IgM相比，在二聚体IgM中观察到Asn-565糖位点的占用水平显著降低，并且在四聚体IgM中没有观察到Asn-565的聚糖占用。糖肽液相色谱-电喷雾串联质谱分析显示，主要是复杂型聚糖与大量的异质性，中性;单唾液酸，二唾液酸和三唾液酸化;岩藻糖基和非岩藻糖基寡糖结合草鱼血清IgM。在单个位点处的聚糖变异在Asn-262糖位点处最大。与其他种属中的IgM不同，在Asn-565糖位点处仅发现痕量复合型聚糖，而未发现高甘露糖聚糖。基质辅助激光解吸电离分析释放的聚糖证实绝大多数碳水化合物的复杂型。这些结果表明，草鱼血清IgM具有独特的N-聚糖特征和高度加工的寡糖连接到单个糖位点。
Immunoglobulin M (IgM) is the major antibody in teleost fish and plays an important role in humoral adaptive immunity. The N-linked carbohydrates presenting on IgM have been well documented in higher vertebrates, but little is known regarding site-specific N-glycan characteristics in teleost IgM. In order to characterize these site-specific N-glycans, we conducted the first study of the N-glycans of each glycosylation site of the grass carp serum IgM. Among the four glycosylation sites, the Asn-262, Asn-303, and Asn-426 residues were efficiently glycosylated, while Asn-565 at the C-terminal tailpiece was incompletely occupied. A striking decrease in the level of occupancy at the Asn-565 glycosite was observed in dimeric IgM compared to that in monomeric IgM, and no glycan occupancy of Asn-565 was observed in tetrameric IgM. Glycopeptide analysis with liquid chromatography-electrospray ionization tandem mass spectrometry revealed mainly complex-type glycans with substantial heterogeneity, with neutral; monosialyl-, disialyl- and trisialylated; and fucosyl-and non-fucosyl-oligosaccharides conjugated to grass carp serum IgM. Glycan variation at a single site was greatest at the Asn-262 glycosite. Unlike IgMs in other species, only traces of complex-type and no high-mannose glycans were found at the Asn-565 glycosite. Matrix-assisted laser desorption ionization analysis of released glycans confirmed the overwhelming majority of carbohydrates were of the complex-type. These results indicate that grass carp serum IgM exhibits unique N-glycan features and highly processed oligosaccharides attached to individual glycosites.
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影响因子：
64.5
作者：
Dennis JW;Nabi IR;Demetriou M
通讯作者：
Demetriou M
影响因子：
4.8
作者：
Kawar, ZS;Van Die, I;Cummings, RD
通讯作者：
Cummings, RD
DOI：
10.1073/pnas.0810163105
发表时间：
2008-12-16
影响因子：
11.1
作者：
Anthony, Robert M.;Wermeling, Fredrik;Ravetch, Jeffrey V.
通讯作者：
Ravetch, Jeffrey V.
影响因子：
16.6
作者：
Harre, Ulrike;Lang, Stefanie C.;Pfeifle, Rene;Rombouts, Yoann;Fruhbeisser, Sabine;Amara, Khaled;Bang, Holger;Lux, Anja;Koeleman, Carolien A.;Baum, Wolfgang;Dietel, Katharina;Groehn, Franziska;Malmstroem, Vivianne;Klareskog, Lars;Kroenke, Gerhard;Kocijan, Roland;Nimmerjahn, Falk;Toes, Rene E. M.;Herrmann, Martin;Scherer, Hans Ulrich;Schett, Georg
通讯作者：
Schett, Georg
影响因子：
20.3
作者：
Kapur, Rick;Kustiawan, Iwan;Vidarsson, Gestur
通讯作者：
Vidarsson, Gestur