Membrane proteins of the nerve growth cone and their developmental regulation
Membrane proteins of the nerve growth cone and their developmental regulation
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神经生长锥膜蛋白及其发育调控
DOI：
10.1523/jneurosci.09-03-01004.1989
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
K. Pfenninger
中科院分区：
文献类型：
--
作者：
P. Simkowitz;L. Ellis;K. Pfenninger
通过亚细胞分级分离从胎鼠脑中分离出的生长锥片段（“生长锥颗粒”，GCP）的膜多肽已被进一步详细分析。通过一维凝胶电泳（Ellis 等人，1985b）检测到的盐洗 GCP 膜的主要多肽在二维凝胶中解析为 52 kDa 的点，与 β-微管蛋白共迁移并与抗 β-微管蛋白反应； [0130] 46kDa，pl 4.3，多肽（pp46)，其在可溶性级分中没有等同物并且与GCP的主要磷蛋白之一（Katz等人，1985)和GAP43（Willard等人，1985)相同；与肌动蛋白共迁移的 42 kDa 点；以及没有可溶性等效物的 34 kDa (p34) 物种。一维凝胶中鉴定出的突出的 38 kDa 双峰很难在二维凝胶中解析。主要磷蛋白 pp80ac、pp46 和 pp40（Katz 等，1985）以及 p34 分配到 Triton X-114 提取物的油相中，表明它们是完整的膜蛋白，至少在我们的实验条件下是这样。这里报道的 pp46 的特性与 GAP43/B50/F1 预测的高度亲水性氨基酸序列相冲突（Basi 等人，1987；Karns 等人，1987）。生长锥蛋白和突触前膜蛋白的比较如下。眼部注射 35S-蛋氨酸后，分别从胎儿和成年大鼠的视神经目标区域分离 GCP 和突触体。通过一维和二维凝胶电泳分离多肽，并通过荧光照相法鉴定放射性标记的物质。标记的 GCP 和突触体多肽的比较表明，GCP 膜的所有 5 种主要考马斯蓝染色多肽（52、46、42、38、34 kDa）在眼部注射后均被强烈标记。然而，在突触体中，这些多肽即使有标记也很弱。相反，出现了一种 28 kDa 的强烈标记的多肽，以及 GCP 中未见的其他几种物种。因此，主要的生长锥膜蛋白受到发育调节，并且神经元多肽的合成和转运到轴突末端的速率在突触发生时发生巨大变化。
The membrane polypeptides of growth cone fragments (“growth cone particles,” GCPs) isolated from fetal rat brain by subcellular fractionation have been analyzed in further detail. The major polypeptides of salt-washed GCP membranes detected by 1-dimensional gel electrophoresis (Ellis et al., 1985b) resolve in 2-dimensional gels as a spot of 52 kDa that comigrates with beta-tubulin and reacts with anti- beta-tubulin; a 46 kDa, pl 4.3, polypeptide (pp46) that has no equivalent in the soluble fraction and is identical to one of the GCP's major phosphoproteins (Katz et al., 1985) and to GAP43 (Willard et al., 1985); a spot of 42 kDa that comigrates with actin; and a species of 34 kDa (p34) without soluble equivalent. The prominent 38 kDa doublet identified in 1-dimensional gels is difficult to resolve in 2- dimensional gels. The major phosphoproteins pp80ac, pp46, and pp40 (Katz et al., 1985), as well as p34 partition into the oil phase of Triton X-114 extracts, suggesting that they are integral membrane proteins, at least in our experimental conditions. The properties of pp46 reported here are in conflict with the highly hydrophilic amino acid sequence predicted for GAP43/B50/F1 (Basi et al., 1987; Karns et al., 1987). Growth-cone and presynaptic membrane proteins are compared as follows. After eye injection of 35S-methionine, GCPs and synaptosomes are isolated from the target areas of optic nerve of fetal and adult rats, respectively. Polypeptides are separated by 1- and 2- dimensional gel electrophoresis and the radiolabeled species identified fluorographically. The comparison of labeled GCP and synaptosome polypeptides shows that all 5 major Coomassie blue-stained polypeptides of GCP membranes (52, 46, 42, 38, 34 kDa) are intensely labeled after eye injection. However, in synaptosomes, these polypeptides are weakly labeled if at all; instead, an intensely labeled polypeptide of 28 kDa, and several additional species not seen in GCPs, have appeared. Therefore, the major growth cone membrane proteins are developmentally regulated, and the rates of synthesis and transport into the axonal ending of neuronal polypeptides change dramatically at the time of synaptogenesis.
DOI：
10.1073/pnas.83.10.3537
发表时间：
1986-05-01
影响因子：
11.1
作者：
MEIRI, KF;PFENNINGER, KH;WILLARD, MB
通讯作者：
WILLARD, MB
47-kDa 蛋白 (F1) 磷酸化的选择性增加与长时程增强直接相关。
DOI：
10.1016/s0163-1047(85)91426-8
发表时间：
1985
期刊：
Behavioral and neural biology
影响因子：
--
作者：
Routtenberg,A;Lovinger,DM
通讯作者：
Lovinger,DM
神经元生长相关蛋白 GAP-43（B-50，F1）：人类和大鼠 mRNA 的神经元特异性、发育调节和区域分布。
影响因子：
2.9
作者：
Neve,RL;Perrone-Bizzozero,NI;Finklestein,S;Zwiers,H;Bird,E;Kurnit,DM;Benowitz,LI
通讯作者：
Benowitz,LI