Next-generation Interactomics: Considerations for the Use of Co-elution to Measure Protein Interaction Networks*
Next-generation Interactomics: Considerations for the Use of Co-elution to Measure Protein Interaction Networks*
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下一代相互作用组学：使用共洗脱测量蛋白质相互作用网络的注意事项*
DOI：
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发表时间：
2019
影响因子：
7
通讯作者：
L. Foster
中科院分区：
文献类型：
--
作者：
Daniela Salas;R. Stacey;Mopelola O Akinlaja;L. Foster
相互作用组研究对于理解细胞过程是必要的，共洗脱方法非常适合相互作用组的同步和全局探索，以及网络生物扰动的评估。这些方法依赖于来自同一复合物的蛋白质在分级过程中迁移在一起的基本思想。我们回顾了不同的分离技术以及用于共洗脱方法的定量和生物信息学方法，并提供了在它们之间进行选择的设计考虑因素。图解摘要亮点 共洗脱作为一种全局相互作用组图谱方法脱颖而出。优点包括全面的蛋白质分析和相互作用组扰动的测量。可以采用不同的分离、定量和生物信息学策略。设计考虑因素很大程度上取决于所研究的系统。了解蛋白质如何相互作用对于理解细胞过程至关重要。在可用的相互作用组作图方法中，共洗脱作为一种本质上同时进行的方法而脱颖而出，并且能够识别样品中检测到的所有蛋白质之间的相互作用。共洗脱方法的一般工作流程包括温和提取蛋白质复合物并将其分离成多个级分，在适当分析时，在同一复合物中结合在一起的蛋白质将显示出相似的共洗脱曲线。在这篇综述中，我们讨论了共洗脱研究中使用的不同分离、定量和生物信息学策略，以及设计这些研究时的重要考虑因素。与其他方法相比，共洗脱的优点使其成为提出涉及相互作用组扰动的问题时的宝贵起点。
Interactome studies are necessary to understand cellular processes and co-elution methods are well suited for the simultaneous and global exploration of the interactome, as well as the assessment of biological perturbations of the network. These methods rely on the fundamental idea that proteins from the same complex migrate together during fractionation. We review the different separation techniques along with the quantification and bioinformatic approaches used for co-elution methods and provide design considerations to choose between them. Graphical Abstract Highlights Co-elution stands out as a global interactome mapping method. Benefits include all-to-all protein analysis and measurement of interactome perturbations. Different separation, quantification and bioinformatic strategies are available. Design considerations depend largely on system under study. Understanding how proteins interact is crucial to understanding cellular processes. Among the available interactome mapping methods, co-elution stands out as a method that is simultaneous in nature and capable of identifying interactions between all the proteins detected in a sample. The general workflow in co-elution methods involves the mild extraction of protein complexes and their separation into several fractions, across which proteins bound together in the same complex will show similar co-elution profiles when analyzed appropriately. In this review we discuss the different separation, quantification and bioinformatic strategies used in co-elution studies, and the important considerations in designing these studies. The benefits of co-elution versus other methods makes it a valuable starting point when asking questions that involve the perturbation of the interactome.
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影响因子：
9.9
作者：
Kastritis PL;O'Reilly FJ;Bock T;Li Y;Rogon MZ;Buczak K;Romanov N;Betts MJ;Bui KH;Hagen WJ;Hennrich ML;Mackmull MT;Rappsilber J;Russell RB;Bork P;Beck M;Gavin AC
通讯作者：
Gavin AC
影响因子：
29
作者：
Heide, Heinrich;Bleier, Lea;Brandt, Ulrich
通讯作者：
Brandt, Ulrich
影响因子：
64.5
作者：
Hein, Marco Y.;Hubner, Nina C.;Mann, Matthias
通讯作者：
Mann, Matthias
DOI：
10.1016/j.chroma.2017.01.082
发表时间：
2017-03-17
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Wagner BM;Schuster SA;Boyes BE;Shields TJ;Miles WL;Haynes MJ;Moran RE;Kirkland JJ;Schure MR
通讯作者：
Schure MR