Functionally distinct T-helper cell phenotypes predict resistance to different types of parasites in a wild mammal
Functionally distinct T-helper cell phenotypes predict resistance to different types of parasites in a wild mammal
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功能上不同的T辅助细胞表型预测野生哺乳动物对不同类型寄生虫的抵抗力
DOI：
10.1101/2021.03.15.435455
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Corripio-Miyar Y
中科院分区：
文献类型：
--
作者：
Corripio-Miyar Y
适应性免疫系统对于脊椎动物对感染的有效反应至关重要，辅助性T（Th）细胞在协调这些反应中起关键作用。在自然人群中，共感染是常态，不同的Th反应可能发挥重要作用，在维持主机的健康和健身，野生动物的关系仍然知之甚少。在这项研究中，我们的特点是在功能上不同的Th反应的野生群体Soay羊计数细胞表达Th亚群特异性转录因子和量化Th相关的细胞因子的变化。我们测试的预测，提高Th 1和Th 2的反应应该预测减少apicomplexan和蠕虫寄生虫负担，分别。所有措施Th相关的细胞因子的生产随着年龄的增长，而Th 17和调节Th相关的细胞因子的生产增加更迅速地随着年龄的男性比女性。独立于年龄、性别和彼此，IL-4和Gata 3负预测胃肠道线虫粪便卵计数，而IFN-γ负预测球虫粪便卵囊计数。我们的研究结果从实验室外提供了重要的支持，即Th 1和Th 2反应预测了对不同种类寄生虫的抗性，并说明了利用实验室免疫学的特定试剂和工具将如何阐明我们对野外宿主-寄生虫相互作用的理解。
The adaptive immune system is critical to an effective response to infection in vertebrates, with T-helper (Th) cells pivotal in orchestrating these responses. In natural populations where co-infections are the norm, different Th responses are likely to play an important role in maintaining host health and fitness, a relationship which remains poorly understood in wild animals. In this study, we characterised variation in functionally distinct Th responses in a wild population of Soay sheep by enumerating cells expressing Th-subset specific transcription factors and quantifying Th-associated cytokines. We tested the prediction that raised Th1 and Th2 responses should predict reduced apicomplexan and helminth parasite burdens, respectively. All measures of Th-associated cytokine production increased with age, while Th17- and regulatory Th-associated cytokine production increased more rapidly with age in males than females. Independent of age, sex, and each other, IL-4 and Gata3 negatively predicted gastro-intestinal nematode faecal egg count, while IFN-γ negatively predicted coccidian faecal oocyst count. Our results provide important support from outside the laboratory that Th1 and Th2 responses predict resistance to different kinds of parasites, and illustrate how harnessing specific reagents and tools from laboratory immunology will illuminate our understanding of host-parasite interactions in the wild.
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影响因子：
7
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
Entrican G