The effects of vasopressin on electrical activity in the guinea‐pig supraoptic nucleus in vitro.
The effects of vasopressin on electrical activity in the guinea‐pig supraoptic nucleus in vitro.
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加压素对体外豚鼠视上核电活动的影响。
DOI：
10.1113/jphysiol.1983.sp014648
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
N. Ogata
中科院分区：
文献类型：
--
作者：
H. Abe;M. Inoue;T. Matsuo;N. Ogata
使用豚鼠下丘脑的脑切片来确定加压素对视上核神经元细胞内电位的影响。加压素（0.05 - 1 i.u./ ml.）使膜去极化，而输入电阻没有明显变化，并降低自发放电率。加压素的这种作用在含有0 mM-Ca 2+、12 mM-Mg 2+和0.3 mM-EGTA的培养基中得以保留。加压素诱导的去极化幅度与电压无关。离子替代实验表明，[K+]o、[Cl-]o和[Ca 2 +]o的变化对加压素引起的去极化幅度影响不大，而[Na+]o的耗竭则使去极化幅度略有降低。加压素诱导的去极化在15 ℃的温度下被10（-4）M剂量的哇巴因阻断。二丁酰环AMP（2 mM）产生的电生理效应与加压素相似，罂粟碱（10（-4）M）或茶碱（10（-2）M）可增强这两种药物的作用。加压素孵育的组织中cAMP含量显著高于正常Krebs液孵育的组织。我们的结论是，加压素直接调节视上神经元的活动，可能通过激活腺苷酸环化酶。
Brain slices of the guinea‐pig hypothalamus were used to determine the effects of vasopressin on intracellular potentials in neurones of the supraoptic nucleus. Vasopressin (0.05‐1 i.u./ml.) depolarized the membrane without apparent change in the input resistance and decreased the spontaneous firing rate. This action of vasopressin was retained in the medium containing 0 mM‐Ca2+, 12 mM‐Mg2+ and 0.3 mM‐EGTA. Amplitude of the vasopressin‐induced depolarization was voltage‐independent. Ion‐substitution experiments showed that the changes in [K+]o, [Cl‐]o and [Ca2+]o had little effect upon the amplitude of vasopressin‐induced depolarization, whereas the depletion of [Na+]o slightly reduced the amplitude. The vasopressin‐induced depolarization was blocked at a temperature of 15 degrees C and by ouabain in a dose of 10(‐4) M. Dibutyryl cyclic AMP (2 mM) produced electrophysiological effects similar to those seen with vasopressin, and actions of both agents were potentiated by either papaverine (10(‐4) M) or theophylline (10(‐2) M). Contents of cyclic AMP in tissues incubated with vasopressin were significantly higher than in cases of incubation with normal Krebs solution. We conclude that vasopressin directly modulates the activity of supraoptic neurones, possibly through activation of adenylate cyclase.
抗利尿激素对大鼠体外肾素分泌抑制作用的钙依赖性。
DOI：
10.1113/jphysiol.1981.sp013729
发表时间：
1981
期刊：
The Journal of physiology
影响因子：
--
作者：
Churchill,PC
通讯作者：
Churchill,PC