Adhesive hydrogel wrap loaded with Netrin-1-modified adipose-derived stem cells: An effective approach against periarterial inflammation after endovascular intervention.
Adhesive hydrogel wrap loaded with Netrin-1-modified adipose-derived stem cells: An effective approach against periarterial inflammation after endovascular intervention.
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DOI：
10.3389/fbioe.2022.944435
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发表时间：
2022
影响因子：
5.7
通讯作者：
Lu, Xinwu
中科院分区：
文献类型：
--
作者：
Jiang, Yihong;Cai, Yuting;Hu, Jiateng;Zhang, Xing;Lei, Jiahao;Peng, Zhaoxi;Huang, Qun;Xu, Zhijue;Li, Bo;Qin, Jinbao;Li, Weimin;Sun, Dazhi;Ye, Kaichuang;Lu, Xinwu
关键词：
血管内介入治疗，如球囊扩张和支架植入，目前被推荐作为外周动脉疾病（PAD）患者的主要治疗方法，大大改善了患者的预后。然而，血管内介入治疗后随之发生的管腔再狭窄已成为一个重要的临床问题。炎症已被证明是术后再狭窄的关键。在以前的研究中，我们已经确定Netrin-1修饰的脂肪干细胞（N-ADSCs）移植是修复血管损伤的有效抗炎策略。然而，如何将N-ADSC持续递送到受损的动脉上仍有待探索。因此，我们开发了一种负载N-ADSC的粘附性双网络（DN）水凝胶包裹物，用于持续的血管周围递送。受贻贝黏附机制的启发，我们开发了一种具有粘性和坚韧的聚丙烯酰胺/海藻酸钙/还原氧化石墨烯/聚多巴胺（PAM/CA/rGO/PDA）水凝胶。多巴胺被连接到石墨烯片并被有限氧化以产生游离儿茶酚基团。该水凝胶可以包裹受损的动脉，并通过N-ADSCs诱导抗炎作用。体外实验表明，N-ADSCs能显著促进巨噬细胞M2极化，并降低炎症因子的表达。在大鼠颈动脉导丝损伤模型中的体内实验表明，负载N-ADSCs的粘附性水凝胶包裹物可以显著减轻动脉炎症，抑制内膜增生并改善再内皮化。总之，这种新开发的N-ADSCs负载水凝胶包裹物提供了一种有效的缓释系统，这可能是预防和治疗血管内介入术后再狭窄的一种有前途的方法。
Endovascular interventions, such as balloon dilation and stent implantation, are currently recommended as the primary treatment for patients with peripheral artery disease (PAD), greatly improving patient prognosis. However, the consequent lumen restenosis that occurs after endovascular interventions has become an important clinical problem. Inflammation has been proven to be crucial to postoperative restenosis. In previous studies we have identified that Netrin-1-modified adipose-derived stem cells (N-ADSCs) transplantation is an effective anti-inflammatory strategy to repair vascular damage. Nevertheless, it remained to be explored how one could constantly deliver N-ADSCs onto damaged arteries. Therefore, we developed an adhesive double network (DN) hydrogel wrap loaded with N-ADSCs for sustained perivascular delivery. Inspired by the adhesion mechanism of mussels, we developed an adhesive and tough polyacrylamide/calcium-alginate/reduced graphene oxide/polydopamine (PAM/CA/rGO/PDA) hydrogel. Dopamine was attached to graphene sheets and limitedly oxidized to generate free catechol groups. The hydrogel could wrap damaged arteries and induce anti-inflammatory effects through N-ADSCs. In vitro experiments demonstrated that N-ADSCs significantly promoted the M2 polarization of macrophages to anti-inflammatory phenotypes and reduced the expression of inflammatory factors. In vivo experiments in a rat carotid artery guidewire injury model showed that the adhesive hydrogel wrap loaded with N-ADSCs could significantly reduce arterial inflammation, inhibit intimal hyperplasia and improve re-endothelialization. Altogether, this newly developed N-ADSCs-loaded hydrogel wrap provides an effective slow-releasing system, which may be a promising way to prevent and treat restenosis after endovascular interventions.
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影响因子：
16.6
作者：
Hong, Yi;Zhou, Feifei;Ouyang, Hongwei
通讯作者：
Ouyang, Hongwei
DOI：
10.1161/circinterventions.113.000195
发表时间：
2013-12
期刊：
Circulation. Cardiovascular interventions
影响因子：
--
作者：
Gasper WJ;Jimenez CA;Walker J;Conte MS;Seward K;Owens CD
通讯作者：
Owens CD
影响因子：
4.3
作者：
Owens, Christopher D.;Gasper, Warren J.;Walker, Joy P.;Alley, Hugh F.;Conte, Michael S.;Grenon, S. Marlene
通讯作者：
Grenon, S. Marlene
Netrin-1 通过改善原位内皮祖细胞功能促进炎症消退，实现小直径组织工程血管的内皮化
DOI：
10.1002/advs.201700278
发表时间：
2017-12
期刊：
Advanced science (Weinheim, Baden-Wurttemberg, Germany)
影响因子：
--
作者：
Li Y;Wan S;Liu G;Cai W;Huo D;Li G;Yang M;Wang Y;Guan G;Ding N;Liu F;Zeng W;Zhu C
通讯作者：
Zhu C
MSC 衍生的外泌体促进 M2 极化并增强皮肤伤口愈合
影响因子：
4.3
作者：
He, Xiaoning;Dong, Zhiwei;Li, Bei
通讯作者：
Li, Bei