Redox reactivity of the binuclear iron active site of porcine purple acid phosphatase (uteroferrin)
Redox reactivity of the binuclear iron active site of porcine purple acid phosphatase (uteroferrin)
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猪紫色酸性磷酸酶（子宫铁蛋白）双核铁活性位点的氧化还原反应性
DOI：
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
A. Sykes
中科院分区：
文献类型：
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作者：
M. A. Aquino;A. Sykes
Redox interconversions (25 °C) of active FeIIFeIII purple acid phosphatase (PAPr), and the inactive FeIIIFeIII form (PAPo), have been explored at pH 5.0 (close to maximum activity), I= 0.100 M (NaCl). At this pH the PAPo–PAPr, couple has a reduction potential E°′ of 367 mV vs. normal hydrogen electrode. Whereas with [Co(phen)3]3+ as oxidant for PAPr first-order dependencies on both reactants are observed (k= 1.26 M–1 s–1), with [Fe(CN)6]3– saturation kinetics are obtained with association. K= 540 M–1, occurring prior to electron transfer, ket= 1.0 s–1. The latter reaction undergoes competitive inhibition with redox inactive [Cr(CN)6]3–(Kcr= 550 M–1) and [Mo(CN)8]4–(KMo= 1580 M–1) consistent with a positively charged locality on the protein surface influencing reactivity. With [Ru(NH3)6]2+ reduction of PAPo is too fast to monitor, but with the less strongly reducing [Ru(NH3)5(H2O)]2+ a rate law first order in both reactants (k= 2.2 × 105 M–1 s–1) is observed. Reactions of both these reductants with the less strongly oxidising phosphate bound PAPo–PO4 form (183 mV) were also studied. On reduction of PAPr with S2O42– a bleaching of the colour is observed consistent with FeIIFeII formation. After 30 min only 40% of the protein could be restored to one or other of the higher oxidation states, indicating loss of FeII.
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铁从子宫铁蛋白（紫色酸性磷酸酶）转移到与酸性磷酸酶活性相关的转铁蛋白。
DOI：
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发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Nuttleman,PR;Roberts,RM
通讯作者：
Roberts,RM
子宫铁蛋白的线性电场效应和电子自旋回波研究。
DOI：
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发表时间：
1985
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Antanaitis,BC;Peisach,J;Mims,WB;Aisen,P
通讯作者：
Aisen,P
还原子宫铁蛋白及其“EPR-沉默”磷酸盐复合物的磁化和电子顺磁共振研究。
DOI：
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发表时间：
1988
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Day,EP;David,SS;Peterson,J;Dunham,WR;Bonvoisin,JJ;Sands,RH;QueJr,L
通讯作者：
QueJr,L
高盐型牛脾紫色酸性磷酸酶的电子顺磁共振研究。
影响因子：
2.9
作者：
Crowder,MW;Vincent,JB;Averill,BA
通讯作者：
Averill,BA
猪子宫和牛脾脏紫色酸性磷酸酶的 1H NMR 和 NOE 研究。
影响因子：
2.9
作者：
Wang,Z;Ming,LJ;QueJr,L;Vincent,JB;Crowder,MW;Averill,BA
通讯作者：
Averill,BA