Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure.
Proteomic analysis of human bronchoalveolar lavage fluid after subsgemental exposure.
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DOI：
10.1021/pr400066g
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发表时间：
2013-05-03
影响因子：
4.4
通讯作者：
Marshall HE
中科院分区：
文献类型：
--
作者：
Foster MW;Thompson JW;Que LG;Yang IV;Schwartz DA;Moseley MA;Marshall HE
通过支气管肺泡灌洗(BAL)采样的呼吸道液体分析，为询问健康和疾病的肺部生物学提供了一种微创途径。在这里，我们使用免疫耗竭，结合凝胶和无标记LC-MS/MS，对在支气管镜下将生理盐水、脂多糖(LPS)或屋尘螨抗原注入三个不同的肺段后从受试者身上回收的样本中的BAL液(BALF)蛋白质组进行定量。在对9个样本进行量化的200多种独特蛋白质中，中性粒细胞颗粒来源的蛋白质和急性时相蛋白质在脂多糖暴露的肺叶中高度浓缩。其中，肽聚糖反应蛋白1被证实为中性粒细胞炎症的新标记物。与之前对同一队列中的呼吸道细胞进行的转录分析相比，BALF蛋白质组揭示了一组新的反应因子。与暴露无关，右肺下叶中气管表达的蛋白质的丰富表明BALF蛋白质组中可能存在构成性叶内变异；对多个肺亚片段的采样似乎也有助于识别在基线水平区分个体的蛋白质特征。总体而言，这项概念验证研究验证了BALF蛋白质组学的强大工作流程，并证明了蛋白质组学和基因组学技术在研究呼吸道(病理)生理学方面的互补性质。
The analysis of airway fluid, as sampled by bronchoalveolar lavage (BAL), provides a minimally invasive route to interrogate lung biology in health and disease. Here, we used immunodepletion, coupled with gel- and label-free LC-MS/MS, for quantitation of the BAL fluid (BALF) proteome in samples recovered from human subjects following bronchoscopic instillation of saline, lipopolysaccharide (LPS) or house dust mite antigen into three distinct lung subsegments. Among more than 200 unique proteins quantified across nine samples, neutrophil granule-derived and acute phase proteins were most highly enriched in the LPS-exposed lobes. Of these, peptidoglycan response protein 1 was validated and confirmed as a novel marker of neutrophilic inflammation. Compared to a prior transcriptomic analysis of airway cells in this same cohort, the BALF proteome revealed a novel set of response factors. Independent of exposure, the enrichment of tracheal-expressed proteins in right lower lung lobes suggests a potential for constitutive intralobar variability in the BALF proteome; sampling of multiple lung subsegments also appears to aid in the identification of protein signatures that differentiate individuals at baseline. Collectively, this proof-of-concept study validates a robust workflow for BALF proteomics and demonstrates the complementary nature of proteomic and genomic techniques for investigating airway (patho)physiology.
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DOI：
10.1177/0968051907086464
发表时间：
2007-01-01
期刊：
JOURNAL OF ENDOTOXIN RESEARCH
影响因子：
--
作者：
Kanayama, Seiji;Yamada, Yoshihiko;Kobayashi, Hiroshi
通讯作者：
Kobayashi, Hiroshi
人类基因表达的组织特异性的综合功能分析。
影响因子：
5.4
作者：
Dezso Z;Nikolsky Y;Sviridov E;Shi W;Serebriyskaya T;Dosymbekov D;Bugrim A;Rakhmatulin E;Brennan RJ;Guryanov A;Li K;Blake J;Samaha RR;Nikolskaya T
通讯作者：
Nikolskaya T
影响因子：
4.4
作者：
Fu, Xiaoyun;Gharib, Sina A.;Heinecke, Jay W.
通讯作者：
Heinecke, Jay W.
影响因子：
2.9
作者：
LENZ, AG;MEYER, B;MAIER, K
通讯作者：
MAIER, K
DOI：
10.1152/ajplung.00129.2003
发表时间：
2004-04-01
影响因子：
4.9
作者：
Lauredo, IT;Forteza, RM;Abraham, WM
通讯作者：
Abraham, WM