Unraveling the cause of white striping in broilers using metabolomics.
Unraveling the cause of white striping in broilers using metabolomics.
复制标题
使用代谢组学揭示肉鸡中白色条纹的原因。
DOI：
10.3382/ps/pey266
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发表时间：
2018-11-01
期刊：
影响因子：
4.4
通讯作者：
Pérez-Bonilla A
中科院分区：
文献类型：
--
作者：
Boerboom G;van Kempen T;Navarro-Villa A;Pérez-Bonilla A
白色条纹病（WS）是影响肉鸡生产的主要问题。受这种肌病影响的鱼片呈现损害肉的质量的病理，并且最重要的是，使鱼片更容易被消费者拒绝。确切的病因仍然未知，这就是为什么对肉鸡的乳房样品进行代谢组学分析的原因。总体目标是确定参与WS发病机制的生物学途径。对总共51个肌肉样本进行分析，并区分正常（n = 19），中度影响（n = 24）和严重影响（n = 8）的乳房鱼片。采用气相色谱质谱分析和液相色谱四极杆飞行时间质谱法对样品进行分析。随后使用各种多变量统计程序对数据进行标准化、归一化和分析。代谢组学允许确定几个途径，改变了白色条纹的乳房鱼片。三羧酸循环表现出相反的方向性。这在文献中被描述为回流，并使TCA循环能够通过基质水平磷酸化产生高能磷酸盐，因此在缺氧条件下产生能量。线粒体脂肪酸氧化受到限制，尤其是顺式-5-14：1肉毒碱的干扰（log 2FC为2，P < 0.01）。因此，有害脂肪酸的积累发生了，特别是长链的，这会破坏细胞结构。精氨酸向瓜氨酸的转化增加，可能是为了产生一氧化氮，在缺氧条件下增强血液流动。然而，一氧化氮也会增加氧化损伤。牛磺酸的增加（log 2FC为1.2，P < 0.05）表明在缺氧条件下肌膜的稳定。最后，有机渗透物（山梨醇，牛磺酸和丙氨酸）增加（P < 0.05），在严重影响的鸟类，这可能会破坏细胞体积的维持。根据本研究的结果，缺氧是肉鸡WS最可能的原因/引发剂。我们推测，患有WS的鸟类在肌肉中具有足以支持生长的血管支持系统，但触发物如活动导致局部缺氧，从而损伤组织。
White striping (WS) is a major problem affecting the broiler industry. Fillets affected by this myopathy present pathologies that compromise the quality of the meat, and most importantly, make the fillets more prone to rejection by the consumer. The exact etiology is still unknown, which is why a metabolomics analysis was performed on breast samples of broilers. The overall objective was to identify biological pathways involved in the pathogenesis of WS. The analysis was performed on a total of 51 muscle samples and distinction was made between normal (n = 19), moderately affected (n = 24) and severely affected (n = 8) breast fillets. Samples were analyzed using gas chromatographic mass spectral analysis and liquid chromatography quadrupole time-of-flight mass spectrometry. Data were subsequently standardized, normalized and analyzed using various multivariate statistical procedures. Metabolomics allowed for the identification of several pathways that were altered in white striped breast fillets. The tricarboxylic acid cycle exhibited opposing directionalities. This is described in literature as the backflux and enables the TCA cycle to produce high-energy phosphates through matrix-level phosphorylation and, therefore, produce energy under conditions of hypoxia. Mitochondrial fatty acid oxidation was limited due to disturbances in especially cis-5–14:1 carnitine (log2 FC of 2, P < 0.01). Because of this, accumulation of harmful fatty acids took place, especially long-chain ones, which damages cell structures. Conversion of arginine to citrulline increased presumably to produce nitric oxide, which enhances blood flow under conditions of hypoxia. Nitric oxide however also increases oxidative damage. Increases in taurine (log2 FC of 1.2, P < 0.05) suggests stabilization of the sarcolemma under hypoxic conditions. Lastly, organic osmolytes (sorbitol, taurine, and alanine) increased (P < 0.05) in severely affected birds; likely this disrupts cell volume maintenance. Based on the results of this study, hypoxia was the most likely cause/initiator of WS in broilers. We speculate that birds suffering from WS have a vascular support system in muscle that is borderline adequate to support growth, but triggers like activity results in local hypoxia that damages tissue.
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影响因子：
4.3
作者：
Kutmon M;van Iersel MP;Bohler A;Kelder T;Nunes N;Pico AR;Evelo CT
通讯作者：
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影响因子：
4.4
作者：
Kuttappan, V. A.;Shivaprasad, H. L.;Owens, C. M.
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影响因子：
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作者：
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作者：
Ferreira, T. Z.;Casagrande, R. A.;Kindlein, L.
通讯作者：
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