Hearing Loss after Round Window Surgery in Mice Is due to Middle Ear Effusion.
Hearing Loss after Round Window Surgery in Mice Is due to Middle Ear Effusion.
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DOI：
10.1159/000449239
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发表时间：
2016
影响因子：
1.6
通讯作者：
Chien WW
中科院分区：
文献类型：
--
作者：
Zhu BZ;Saleh J;Isgrig KT;Cunningham LL;Chien WW
Delivery of therapeutic agents directly through the round window (RW) offers promise for treating sensorineural hearing loss. However, hearing loss can result from the surgical approach itself, and the reasons for this are poorly understood. We examined the hearing loss following the three major steps involved with the RW approach to access the mouse cochlea: bullostomy, RW puncture, and RW injection. 21 adult CBA/J mice underwent bullostomy alone; 10 underwent RW puncture, and 8 underwent RW injection with PBS with 5% glycerol. Auditory brainstem responses and otoscopy were performed preoperatively and up to six weeks postoperatively. Hair cells were stained and survival was assessed using immunofluorescence. One week postoperatively, mice in all groups showed significant threshold shifts. Otoscopy revealed approximately half of all mice had middle ear effusion (MEE), with a higher incidence of effusion in the RW puncture and RW injection groups. Those with MEE had significant ABR threshold shifts, whereas those without MEE had minimal hearing loss. MEE persisted through six weeks in a majority of cases, but in those mice with MEE resolution, there was at least partial improvement in hearing. Immunohistochemistry showed minimal loss of hair cells in all animals. MEE is highly correlated with hearing loss in mice undergoing round window surgery. Otoscopy is an important adjunct to consider after ear surgery in mice, as MEE may contribute to post-surgical hearing loss.
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影响因子：
12.4
作者：
Kawamoto, K;Oh, SH;Raphael, Y
通讯作者：
Raphael, Y
DOI：
10.1126/science.1241062
发表时间：
2014-05-09
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Géléoc GS;Holt JR
通讯作者：
Holt JR
影响因子：
1.2
作者：
Akil, Omar;Rouse, Stephanie L.;Lustig, Lawrence R.
通讯作者：
Lustig, Lawrence R.
影响因子：
2.8
作者：
STEEL, KP;MOORJANI, P;BOCK, GR
通讯作者：
BOCK, GR
影响因子：
2.8
作者：
Zheng, Qing Yin;Tong, Yi-Cai Isaac;Yu, Heping
通讯作者：
Yu, Heping