Functional metagenomics unveils a multifunctional glycosyl hydrolase from the family 43 catalysing the breakdown of plant polymers in the calf rumen.
Functional metagenomics unveils a multifunctional glycosyl hydrolase from the family 43 catalysing the breakdown of plant polymers in the calf rumen.
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DOI：
10.1371/journal.pone.0038134
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Golyshin PN
中科院分区：
文献类型：
--
作者：
Ferrer M;Ghazi A;Beloqui A;Vieites JM;López-Cortés N;Marín-Navarro J;Nechitaylo TY;Guazzaroni ME;Polaina J;Waliczek A;Chernikova TN;Reva ON;Golyshina OV;Golyshin PN
来自牛瘤胃的微生物群落以其以高速率降解多种植物聚合物的能力而闻名。在这项工作中，我们确定了15水解酶通过活性为中心的宏基因组分析的纤维粘附微生物群落从奶牛瘤胃。其中7个糖基水解酶和1个阿魏酸酯酶已成功克隆、表达、纯化和鉴定。最引人注目的结果是GH家族43（GHF 43）的蛋白质，以下命名为R_09-02，其具有与该家族中的其它蛋白质非常不同的特征，具有单功能β-木糖苷酶、α-木聚糖酶、α-L-阿拉伯糖酶和α-L-阿拉伯呋喃糖苷酶活性。R_09-02是第一个具有β-1，4木糖苷酶、α-1，5阿拉伯呋喃（pyr）糖苷酶、β-1，4乳糖酶、α-1，6棉子糖酶、α-1，6水苏糖酶、β-半乳糖苷酶和α-1，4葡萄糖苷酶活性的多功能酶。R_09-02蛋白似乎来源于梭菌属（Clostridia）的一个成员的染色体，梭菌属是厚壁菌门（Firmicutes）的一类，其成员在瘤胃环境中高度丰富。R_09-02的进化被认为是由木糖和阿拉伯糖特异性活性（GHF 43成员的典型活性）驱动的，朝向对木质纤维素中含葡萄糖和半乳糖组分的更广泛特异性。来自GHF 43家族的酶利用含木糖、阿拉伯糖、葡萄糖和半乳糖的寡糖的明显能力迄今为止被基因组和宏基因组测序数据分析所忽略，或不能从基因组和宏基因组测序数据分析预测。考虑到丰富的GHF 43编码基因序列在瘤胃（高达7%的所有GH-基因）和多功能表型在此描述，我们的研究结果表明，这个GH家族在木质纤维素物质的消化的生态作用，应显着重新考虑。
Microbial communities from cow rumen are known for their ability to degrade diverse plant polymers at high rates. In this work, we identified 15 hydrolases through an activity-centred metagenome analysis of a fibre-adherent microbial community from dairy cow rumen. Among them, 7 glycosyl hydrolases (GHs) and 1 feruloyl esterase were successfully cloned, expressed, purified and characterised. The most striking result was a protein of GH family 43 (GHF43), hereinafter designated as R_09-02, which had characteristics very distinct from the other proteins in this family with mono-functional β-xylosidase, α-xylanase, α-L-arabinase and α-L-arabinofuranosidase activities. R_09-02 is the first multifunctional enzyme to exhibit β-1,4 xylosidase, α-1,5 arabinofur(pyr)anosidase, β-1,4 lactase, α-1,6 raffinase, α-1,6 stachyase, β-galactosidase and α-1,4 glucosidase activities. The R_09-02 protein appears to originate from the chromosome of a member of Clostridia, a class of phylum Firmicutes, members of which are highly abundant in ruminal environment. The evolution of R_09-02 is suggested to be driven from the xylose- and arabinose-specific activities, typical for GHF43 members, toward a broader specificity to the glucose- and galactose-containing components of lignocellulose. The apparent capability of enzymes from the GHF43 family to utilise xylose-, arabinose-, glucose- and galactose-containing oligosaccharides has thus far been neglected by, or could not be predicted from, genome and metagenome sequencing data analyses. Taking into account the abundance of GHF43-encoding gene sequences in the rumen (up to 7% of all GH-genes) and the multifunctional phenotype herein described, our findings suggest that the ecological role of this GH family in the digestion of ligno-cellulosic matter should be significantly reconsidered.
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影响因子：
5.7
作者：
Jamal-Talabani, S;Boraston, AB;Davies, GJ
通讯作者：
Davies, GJ
DOI：
10.1073/pnas.0806191105
发表时间：
2009-02-10
影响因子：
11.1
作者：
Brulc, Jennifer M.;Antonopoulos, Dionysios A.;White, Bryan A.
通讯作者：
White, Bryan A.
影响因子：
5.1
作者：
Ferrer, M;Golyshina, OV;Golyshin, PN
通讯作者：
Golyshin, PN
影响因子：
6.3
作者：
Li LL;McCorkle SR;Monchy S;Taghavi S;van der Lelie D
通讯作者：
van der Lelie D
影响因子：
3.9
作者：
Brunzelle, Joseph S.;Jordan, Douglas B.;Wawrzak, Zdzislaw
通讯作者：
Wawrzak, Zdzislaw