No evidence for transient transformation via pollen magnetofection in several monocot species
No evidence for transient transformation via pollen magnetofection in several monocot species
复制标题
没有证据表明几种单子叶植物通过花粉磁力转染进行瞬时转化
DOI：
10.1038/s41477-020-00798-6
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发表时间：
2020
期刊：
影响因子：
18
通讯作者：
Fowler, John E.
中科院分区：
文献类型：
--
作者：
Vejlupkova, Zuzana;Warman, Cedar;Sharma, Rita;Scheller, Henrik Vibe;Mortimer, Jenny C.;Fowler, John E.
自然植物试验表明，阳性对照生物法与阴性对照和磁感应方案(两者均为P<；10−6)之间存在统计上的显著差异。总之，使用赵等人描述的方法，我们无法复制任何通过磁感应在百合花粉中进行瞬时转化的证据。虽然我们不能排除在非常罕见的频率下进行瞬时转化的可能性，但我们怀疑，百合花粉磁感应后~90%的瞬时表达效率是由于内源GUS活性，而不是用于评估磁感应成功的GUS报告。最后，尽管我们在百合、高粱和玉米上进行了广泛的试验，但仍未取得成功，因此我们也无法提供证据支持花粉磁感应在单子叶之间广泛适用的想法。我们注意到，我们不能解决花粉磁分离用于稳定转化的用途，因为我们还没有测试报告DNA的基因组整合。然而，考虑到我们的结果，我们认为对于在双子叶植物中工作的群体，特别是那些被引用为成功转化的物种(棉花、胡椒和南瓜)来说，提供关于通过花粉磁感应复制瞬时和稳定转化成功的尝试的数据是重要的。
MATTERS ARISING NATURE PLANTS trials indicates a statistically significant difference between the positive control biolistic method and both the negative control and the magnetofection protocol (P< 10− 6 for both). In conclusion, using the protocol described by Zhao et al., we were unable to reproduce any evidence of transient transformation in lily pollen via magnetofection. Although we cannot rule out transient transformation at very rare frequencies, we suspect that the report of~ 90% transient expression efficiency following the magnetofection of lily pollen was due to endogenous GUS activity, rather than that from the GUS reporter used for assessing magnetofection success. Finally, given our lack of success in lily, sorghum and maize despite extensive trials, we also could not generate evidence to support the idea of broad applicability for pollen magnetofection across monocots. We note that we cannot address the utility of pollen magnetofection for stable transformation, as we have not tested for genomic integration of reporter DNA. However, given our results, we believe that it is important for groups working in dicot species, particularly those cited as successfully transformed (cotton, pepper and pumpkin), to provide data regarding attempts to replicate both transient and stable transformation success via pollen magnetofection.
转基因和非转基因植物雄配子体发育过程中的β-葡萄糖醛酸酶活性
DOI：
--
发表时间：
1989
期刊：
Molecular and General Genetics MGG
影响因子：
--
作者：
L. Plegt;R. Bino
通讯作者：
R. Bino